洗手剂抗巨细胞病毒的比较疗效

Comparative efficacy of handwashing agents against cytomegalovirus.

作者信息

Faix R G

出版信息

Infect Control. 1987 Apr;8(4):158-62. doi: 10.1017/s0195941700065826.

DOI:10.1017/s0195941700065826

Abstract

Conscientious handwashing is often recommended as an important method for limiting transmission of cytomegalovirus (CMV) from infected individuals to health, education, and child care professionals. To assess the efficacy of handwashing, fingertips of radiation-sterilized latex gloves were inoculated with 0.2 mL of ten different CMV strains. Virus in each inoculum was quantitated by plaque assay. After five minutes, viral inocula were allowed to remain (control), or were washed away by dropwise application of 10 mL of distilled water (DI), 5 mL of 0.08% soap followed by 5 mL of DI, 5 mL of 0.01% chlorhexidine gluconate followed by 5 mL of DI, or 5 mL of 0.025% povidone-iodine solution followed by 5 mL of DI. Separate glove fingertips were sampled 5, 15, 30, 60, 120 and 240 minutes after washing and cultured in duplicate for CMV. Similar studies were performed using human cadaver skin. Ordinary soap was as effective at preventing CMV recovery as other more expensive agents. For inocula with less than 5 log10 pfu CMV/mL, washing with water alone was as effective as other agents. This was confirmed in similar studies with human hands using five CMV stains. Handwashing is probably an effective method for removing CMV from contaminated hands.

摘要

认真洗手常被推荐为限制巨细胞病毒（CMV）从感染者传播给健康、教育及儿童保育专业人员的重要方法。为评估洗手的效果，将0.2 mL的十种不同CMV毒株接种到经辐射灭菌的乳胶手套指尖上。通过噬斑测定法对每个接种物中的病毒进行定量。五分钟后，让病毒接种物保留（对照），或者通过逐滴加入10 mL蒸馏水（DI）、5 mL 0.08%肥皂然后再加入5 mL DI、5 mL 0.01%葡萄糖酸氯己定然后再加入5 mL DI，或5 mL 0.025%聚维酮碘溶液然后再加入5 mL DI将其冲洗掉。在洗手后5、15、30、60、120和240分钟对单独的手套指尖进行采样，并一式两份培养CMV。使用人类尸体皮肤进行了类似研究。普通肥皂在防止CMV检出方面与其他更昂贵的制剂一样有效。对于CMV含量低于5 log10 pfu/mL的接种物，仅用水冲洗与其他制剂一样有效。这在使用五种CMV毒株对人手进行的类似研究中得到了证实。洗手可能是一种从污染的手上清除CMV的有效方法。