使用市售试剂盒中的单克隆抗体F6b通过间接免疫荧光法快速诊断巨细胞病毒。
Rapid diagnosis of cytomegalovirus by indirect immunofluorescence assay with monoclonal antibody F6b in a commercially available kit.
作者信息
Lucas G, Seigneurin J M, Tamalet J, Michelson S, Baccard M, Delagneau J F, Deletoille P
机构信息
Diagnostics Pasteur, Marnes la Coquette, France.
出版信息
J Clin Microbiol. 1989 Feb;27(2):367-9. doi: 10.1128/jcm.27.2.367-369.1989.
Abstract
Anti-human cytomegalovirus (CMV) monoclonal antibody (MAb) F6b produced by clone 95/12 was used for the rapid diagnosis (16 to 24 h) of CMV isolates in 308 clinical specimens and compared with classic isolation. MAb F6b gave 100% correlation with isolation. When this MAb was used for direct diagnosis with 212 urine and bronchoalveolar specimens, detection of CMV varied from 50 to 75% compared with viral isolation.
摘要
由克隆95/12产生的抗人巨细胞病毒（CMV）单克隆抗体（MAb）F6b用于308份临床标本中CMV分离株的快速诊断（16至24小时），并与传统分离方法进行比较。MAb F6b与分离结果的相关性为100%。当使用该单克隆抗体对212份尿液和支气管肺泡标本进行直接诊断时，与病毒分离相比，CMV的检测率在50%至75%之间。
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