a A. N. Belozersky Institute of Physico-Chemical Biology , Lomonosov Moscow State University , Moscow , Russia.
b Physical Department , Lomonosov Moscow State University , Moscow , Russia.
Cell Adh Migr. 2019 Dec;13(1):98-105. doi: 10.1080/19336918.2018.1540903. Epub 2018 Oct 29.
Neutrophils release branched-chain (valine, isoleucine, leucine), aromatic (tyrosine, phenylalanine) and positively charged free amino acids (arginine, ornithine, lysine, hydroxylysine, histidine) when adhere and spread onto fibronectin. In the presence of agents that impair cell spreading or adhesion (cytochalasin D, fMLP, nonadhesive substrate), neutrophils release the same amino acids, except for a sharp decrease in hydroxylysine and an increase in phenylalanine, indicating their special connection with cell adhesion. Plasma of patients with diabetes is characterized by an increased content of branched-chain and aromatic amino acids and a reduced ratio of arginine/ornithine compared to healthy human plasma. Our data showed that the secretion of neutrophils, regardless of their adhesion state, can contribute to this shift in the amino acid content. Abbreviations: BCAAs: branched-chain amino acids; Е2: 17β-estradiol; LPS: lipopolysaccharide from Salmonella enterica serovar Typhimurium; fMLP: N-formylmethionyl-leucyl-phenylalanine.
中性粒细胞在黏附和铺展到纤维连接蛋白上时会释放支链（缬氨酸、异亮氨酸、亮氨酸）、芳香族（酪氨酸、苯丙氨酸）和带正电荷的游离氨基酸（精氨酸、鸟氨酸、赖氨酸、羟赖氨酸、组氨酸）。在细胞铺展或黏附受到抑制的情况下（细胞松弛素 D、fMLP、非黏附性底物），中性粒细胞也会释放相同的氨基酸，但羟赖氨酸含量急剧下降，苯丙氨酸含量增加，这表明它们与细胞黏附有特殊的联系。糖尿病患者的血浆中支链和芳香族氨基酸的含量增加，精氨酸/鸟氨酸的比值降低，与健康人血浆相比。我们的数据表明，中性粒细胞的分泌，无论其黏附状态如何，都可能导致氨基酸含量的这种变化。缩写：BCAAs：支链氨基酸；E2：17β-雌二醇；LPS：鼠伤寒沙门氏菌脂多糖；fMLP：N-甲酰基甲硫氨酰亮氨酰苯丙氨酸。
