Suzuki Youhei, Shiotsuki Takahiro, Jouraku Akiya, Miura Ken, Minakuchi Chieka
Graduate School of Bio-Agricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
National Agriculture and Food Research Organization, Tsukuba 305-8634, Japan.
J Pestic Sci. 2017 Aug 20;42(3):93-96. doi: 10.1584/jpestics.D17-023.
We examined the susceptibility of field strains (BO-1, BO-2, TO-1, and YH-1) and one laboratory strain (H-1) of the western flower thrip, , to benzoylureas. LC values of novaluron were determined as 0.64 ppm against laboratory strain and 2.1-130 ppm against field strains. In the presence of piperonyl butoxide, a cytochrome P450 inhibitor, the insecticidal activity of novaluron tended to be enhanced. To examine whether point mutations in () discovered in an etoxazole-resistant strain of and a benzoylurea-resistant strain of exist in , the nucleotide sequence of was analyzed. We found a nonsynonymous substitution that corresponded to the location of the mutations found in and in the field strains of but not in the laboratory strain, indicating that this point mutation might be associated with the benzoylurea resistance exhibited by the field strains.
我们检测了西花蓟马的田间菌株（BO - 1、BO - 2、TO - 1和YH - 1）以及一株实验室菌株（H - 1）对苯甲酰脲类药剂的敏感性。测定了虱螨脲对实验室菌株的LC值为0.64 ppm，对田间菌株的LC值为2.1 - 130 ppm。在细胞色素P450抑制剂胡椒基丁醚存在的情况下，虱螨脲的杀虫活性有增强的趋势。为了检测在唑虫酰胺抗性菌株和苯甲酰脲抗性菌株中发现的（）点突变是否存在于西花蓟马中，我们分析了的核苷酸序列。我们发现在西花蓟马的田间菌株中存在一个非同义替换，其位置与在和中发现的突变位置相对应，但在实验室菌株中未发现，这表明该点突变可能与田间菌株表现出的苯甲酰脲抗性有关。
