一种新型四环素反应性转基因小鼠品系，用于骨骼肌特异性基因表达。

A novel tetracycline-responsive transgenic mouse strain for skeletal muscle-specific gene expression.

机构信息

The Center for Muscle Biology, University of Kentucky, Lexington, KY, 40536, USA.

Department of Physiology, College of Medicine, University of Kentucky, 800 Rose Street, Medical Science Building, Rm: MS-607A, Lexington, KY, 40536, USA.

出版信息

Skelet Muscle. 2018 Oct 27;8(1):33. doi: 10.1186/s13395-018-0181-y.

BACKGROUND

The tetracycline-responsive system (Tet-ON/OFF) has proven to be a valuable tool for manipulating gene expression in an inducible, temporal, and tissue-specific manner. The purpose of this study was to create and characterize a new transgenic mouse strain utilizing the human skeletal muscle α-actin (HSA) promoter to drive skeletal muscle-specific expression of the reverse tetracycline transactivator (rtTA) gene which we have designated as the HSA-rtTA mouse.

METHODS

To confirm the HSA-rtTA mouse was capable of driving skeletal muscle-specific expression, we crossed the HSA-rtTA mouse with the tetracycline-responsive histone H2B-green fluorescent protein (H2B-GFP) transgenic mouse in order to label myonuclei.

RESULTS

Reverse transcription-PCR confirmed skeletal muscle-specific expression of rtTA mRNA, while single-fiber analysis showed highly effective GFP labeling of myonuclei in both fast- and slow-twitch skeletal muscles. Pax7 immunohistochemistry of skeletal muscle cross-sections revealed no appreciable GFP expression in satellite cells.

CONCLUSIONS

The HSA-rtTA transgenic mouse allows for robust, specific, and inducible gene expression across muscles of different fiber types. The HSA-rtTA mouse provides a powerful tool to manipulate gene expression in skeletal muscle.

背景

四环素反应系统（Tet-ON/OFF）已被证明是一种非常有用的工具，可用于以诱导、时间和组织特异性的方式操纵基因表达。本研究的目的是创建和表征一种新的转基因小鼠品系，利用人骨骼肌α-肌动蛋白（HSA）启动子驱动反向四环素转录激活剂（rtTA）基因在骨骼肌中的特异性表达，我们将其命名为 HSA-rtTA 小鼠。