a Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratory , Richland , WA , USA.
b Biological Sciences Division , Pacific Northwest National Laboratory , Richland , WA , USA.
Expert Rev Proteomics. 2018 Nov;15(11):865-871. doi: 10.1080/14789450.2018.1537787. Epub 2018 Oct 30.
Nanoproteomics, which is defined as quantitative proteome profiling of small populations of cells (<5000 cells), can reveal critical information related to rare cell populations, hard-to-obtain clinical specimens, and the cellular heterogeneity of pathological tissues. Areas covered: We present a brief review of the recent technological advances in nanoproteomics. These advances include new technologies or approaches covering major areas of proteomics workflow ranging from sample isolation, sample processing, high-resolution separations, to MS instrumentation. Expert commentary: We comment on the current state of nanoproteomics and discuss perspectives on both future technological directions and potential enabling applications.
纳米蛋白质组学被定义为对数量较少的细胞（<5000 个细胞）进行定量蛋白质组分析，它可以揭示与稀有细胞群体、难以获得的临床标本以及病理组织的细胞异质性相关的关键信息。
我们简要回顾了纳米蛋白质组学的最新技术进展。这些进展包括涵盖蛋白质组学工作流程主要领域的新技术或方法，包括从样品分离、样品处理、高分辨率分离到 MS 仪器。
我们对纳米蛋白质组学的现状进行了评论，并讨论了未来技术方向和潜在应用的前景。
