李属家族 1 UDP 糖基转移酶的比较基因组学和转录组学分析。

Comparative Genomic and Transcriptomic Analyses of Family-1 UDP Glycosyltransferase in Prunus Mume.

机构信息

Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China.

出版信息

Int J Mol Sci. 2018 Oct 29;19(11):3382. doi: 10.3390/ijms19113382.

Glycosylation mediated by Family-1 UDP-glycosyltransferases (UGTs) plays crucial roles in plant growth and adaptation to various stress conditions. is an ideal crop for analyzing flowering for its early spring flowering characteristics. Revealing the genomic and transcriptomic portfolio of the UGT family in , a species in which have not yet been investigated, is therefore important. In this study, 130 putative genes were identified and phylogenetically clustered into 14 groups. These were distributed unevenly across eight chromosomes and 32 tandem duplication and 8 segmental duplication pairs were revealed. A highly conserved intron insertion event was revealed on the basis of intron/exon patterns within . According to RNA-seq data, these were specifically expressed in different tissues and during the bud dormancy process. In addition, we confirmed the differential expression of some representative genes in response to abscisic acid treatment. Our results will provide important information on the UGT family in that should aid further characterization of their biological roles in response to environmental stress.

家族 1 UDP-糖基转移酶（UGTs）介导的糖基化在植物生长和适应各种胁迫条件中起着至关重要的作用。是分析开花的理想作物，因为它具有早春开花的特点。因此，揭示尚未在其中进行研究的物种中的 UGT 家族的基因组和转录组组合是很重要的。在这项研究中，鉴定了 130 个推定的基因，并根据系统发育聚类为 14 组。这些不均匀地分布在 8 条染色体上，揭示了 32 对串联重复和 8 对片段重复对。根据内含子/外显子模式，在中揭示了一个高度保守的内含子插入事件。根据 RNA-seq 数据，这些在不同组织和芽休眠过程中特异性表达。此外，我们还证实了一些代表性基因在响应脱落酸处理时的差异表达。我们的研究结果将为中的 UGT 家族提供重要信息，这有助于进一步表征它们在应对环境胁迫时的生物学作用。