Cawley J C, Burns G F, Nash T A, Higgy K E, Child J A, Roberts B E
Blood. 1978 Jan;51(1):61-9.
A case of clinically and hematologically typical hairy-cell leukemia has been presented in which, at the various times of testing, 52%-95% of peripheral blood and 73% of splenic mononuclear cells formed spontaneous sheep erythrocyte (E) rosettes. Many of the rosetting cells were shown to be typical morphologic hairy cells by light and electron microscopy. It was found that 70%-75% of peripheral blood mononuclear cells stained with an anti-T antiserum, and this antiserum also abolished E-rosette formation. A variable percentage of peripheral blood mononuclear cells was also shown to bear surface (IgDK) and internal (IgMK and IgGK) immunoglobulins. Additional B-cell features demonstrated included possession of the P29/34 la-like antigen and formation of mouse rosettes. It was demonstrated by a variety of blocking and inhibition studies that the E-rosette formation was not attributable to chance antigen specificity of the surface membrane immunoglobulin. These marker studies suggest that this is a case of true hybrid cell HCL. Despite these unusual marker characteristics, the patient showed no distinctive clinical or hematologic features.
本文报告了一例临床和血液学表现典型的毛细胞白血病病例，在不同检测时间，外周血的52%-95%以及脾单个核细胞的73%形成了自发性绵羊红细胞（E）花环。通过光镜和电镜观察，许多形成花环的细胞为典型形态的毛细胞。发现70%-75%的外周血单个核细胞被抗T抗血清染色，且该抗血清也消除了E花环的形成。外周血单个核细胞的可变百分比也显示带有表面（IgDK）和内部（IgMK和IgGK）免疫球蛋白。显示的其他B细胞特征包括拥有P29/34 la样抗原和形成小鼠花环。通过多种阻断和抑制研究表明，E花环的形成并非归因于表面膜免疫球蛋白偶然的抗原特异性。这些标记研究提示这是一例真正的杂交细胞型毛细胞白血病。尽管有这些不寻常的标记特征，但该患者并无独特的临床或血液学特征。
