Institute for Sustainable Agriculture, Spanish Council for Scientific Research (IAS-CSIC), Alameda del Obispo s/n, Cordoba, Spain.
Centre of Novel Agricultural Products (CNAP), Department of Biology, University of York, York, United Kingdom.
PLoS One. 2018 Nov 7;13(11):e0205880. doi: 10.1371/journal.pone.0205880. eCollection 2018.
The global production of cereal straw as an agricultural by-product presents a significant source of biomass, which could be used as feedstock for the production of second generation biofuels by fermentation. The production of sugars for fermentation is an important measure of straw quality and in its suitability for biofuel production. In this paper, we present a characterization of straw digestibility from a wide range of cereal. Our main objective is to evaluate the variability of fermentable sugars released from different species including wheat (Triticum durum L., Triticum aestivum L.), barley (Hordeum vulgare L.) and triticale (X Triticosecale Wittmack). To this end, we adapted a saccharification method (IAS Method) capable of detecting significant differences of released sugars between cultivars and species, while using separately another method that would serve as a control and with which we could contrast our results (CNAP method). ANOVA analyses revealed that barley has a higher saccharification potential than wheat and triticale and shows more variation between genotypes. Thus, populations derived from crosses among them such as Steptoe × Morex and OWB Dominant × OWB Recessive hold potential for the identification of genetic basis for saccharification-related traits. The correlation of glucose released between the two methods was moderate (R2 = 0.57). An evaluation of the inter- and intra- specific correlation between a number of chemical and agronomical parameters and saccharification suggests that the cell wall thickness and lignin content in straw could be used in breeding programs for the improvement of the saccharification potential. Finally, the lack of correlation between grain yield and saccharification suggests that it would be possible to make a selection of genotypes for dual purpose, low recalcitrance and grain yield.
谷物秸秆作为农业副产品，在全球范围内的产量巨大，是一种潜在的生物质资源，可以作为发酵生产第二代生物燃料的原料。发酵生产糖是衡量秸秆质量和生物燃料生产适用性的重要指标。本文对来自多种谷物的秸秆消化率进行了特征描述。我们的主要目的是评估不同物种（包括小麦、大麦和黑小麦）释放的可发酵糖的变异性。为此，我们采用了一种糖化方法（IAS 方法），该方法能够检测不同品种和物种之间释放糖的显著差异，同时使用另一种方法作为对照，以便与我们的结果进行对比（CNAP 方法）。方差分析表明，大麦的糖化潜力高于小麦和黑小麦，且基因型之间的差异更大。因此，来自它们之间杂交的种群，如 Steptoe×Morex 和 OWB Dominant×OWB Recessive，可能具有与糖化相关性状的遗传基础。两种方法之间释放的葡萄糖相关性中等（R2=0.57）。对一些化学和农艺参数与糖化之间的种间和种内相关性的评估表明，秸秆细胞壁厚度和木质素含量可以用于改良糖化潜力的选育计划。最后，糖化与籽粒产量之间缺乏相关性表明，有可能对低抗逆性和高籽粒产量的基因型进行双重选择。
