Brake S C, Hutchings D E, Morgan B, Lasalle E, Shi T
Neurotoxicol Teratol. 1987 Jan-Feb;9(1):45-9. doi: 10.1016/0892-0362(87)90069-9.
Either 15 or 50 mg/kg delta-9-tetrahydrocannabinol (THC) in sesame oil was administered by gastric intubation to gravid rats during the last two weeks of gestation. A pair-fed control group was administered the vehicle alone and allowed to eat and drink only the amount consumed by the 50 mg/kg group on the same gestation days. A nontreated control group was left undisturbed during pregnancy. All treated and control litters were fostered at birth to untreated dams. Intact litters from the two THC treated and the two control groups were tested at 3-day intervals from birth to 32 days of age for differences in locomotor activity. In addition, pups were tested for nipple attachment on days 2, 5, 8, 11, and 14 of age. There were no differences in locomotor activity among any of the groups although activity level varied during development. Pups from dams exposed to 50 mg/kg of THC, as well as the pair-fed controls, displayed significantly longer latencies to attach to a nipple. These results suggest that the impaired nipple attachment observed among the high-dose offspring was not a primary effect of THC, but rather was secondary to the significant reduction of food and water intake among the dams. The behavioral findings are discussed with respect to other animal and clinical reports of prenatal cannabinoid exposure.
在妊娠的最后两周，通过胃管向妊娠大鼠给予芝麻油中15或50毫克/千克的delta-9-四氢大麻酚（THC）。一对配对喂养的对照组仅给予赋形剂，并仅允许其在相同妊娠天数内进食和饮水与50毫克/千克组消耗的量相同。一个未处理的对照组在怀孕期间不受到干扰。所有处理组和对照组的幼崽在出生时都寄养给未处理的母鼠。对来自两个THC处理组和两个对照组的完整幼崽从出生到32日龄每隔3天测试一次运动活动差异。此外，在幼崽2日龄、5日龄、8日龄、11日龄和14日龄时测试其对乳头的附着情况。尽管在发育过程中活动水平有所变化，但各实验组之间在运动活动方面没有差异。暴露于50毫克/千克THC的母鼠所生的幼崽以及配对喂养的对照组幼崽，在附着到乳头上的潜伏期显著更长。这些结果表明，在高剂量组后代中观察到的乳头附着受损不是THC的主要作用，而是继发于母鼠食物和水摄入量的显著减少。结合其他关于产前大麻素暴露的动物和临床报告对行为学研究结果进行了讨论。
