College of Chemistry and Environmental Science, Qujing Normal University, Qujing, 655011, People's Republic of China.
J Nat Med. 2019 Jan;73(1):312-317. doi: 10.1007/s11418-018-1264-0. Epub 2018 Nov 9.
Illigera aromatica S. Z. Huang et S. L. Mo and Illigera henryi W. W. Sm., belonging to the genus Illigera (Hernandiaceae), are used as herbal medicines for promoting blood circulation and treating tuberculosis. Actinodaphnine, the major bioactive alkaloid, plays an important role in the quality controls of the herbs. In the present study, a rapid, simple, accurate, and precise proton quantitative nuclear magnetic resonance (H-qNMR) method was developed to determine the content of actinodaphnine in I. aromatica and I. henryi. DMSO-d enabled satisfactory separation of the signals to be integrated in H NMR spectrum. 1,4-Dinitrobenzene was selected as an internal standard. The limits of determination and quantitation were 0.005 and 0.038 mg/mL, respectively. This work implied that H-qNMR represents a feasible alternative to HPLC-based methods for quantitation of actinodaphnine in I. aromatica and I. henryi and is suitable for the quality control of I. aromatica and I. henryi.
芳香胡颓子和亨利胡颓子，属于胡颓子属（胡颓子科），被用作草药来促进血液循环和治疗肺结核。作为主要生物活性生物碱的醇式千金藤啶碱在草药质量控制中起着重要作用。在本研究中，开发了一种快速、简单、准确和精密的质子定量核磁共振（H-qNMR）方法，用于测定芳香胡颓子和亨利胡颓子中醇式千金藤啶碱的含量。DMSO-d 能够实现 NMR 谱中可积分信号的令人满意的分离。1,4-二硝基苯被选为内标。检测限和定量限分别为 0.005 和 0.038mg/mL。这项工作表明，H-qNMR 代表了一种可行的替代 HPLC 方法，可用于定量测定芳香胡颓子和亨利胡颓子中的醇式千金藤啶碱，并且适合芳香胡颓子和亨利胡颓子的质量控制。
