Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore; Singapore 117596, Singapore.
Biomolecules. 2018 Nov 16;8(4):151. doi: 10.3390/biom8040151.
Over the last two decades, lipids have come to be understood as far more than merely components of cellular membranes and forms of energy storage, and are now also being implicated to play important roles in a variety of diseases, with lipid biomarker research one of the most widespread applications of lipidomic techniques both in research and in clinical settings. Stable isotope labelling has become a staple technique in the analysis of small molecule metabolism and dynamics, as it is the only experimental setup by which biosynthesis, remodelling and degradation of biomolecules can be directly measured. Using state-of-the-art analytical technologies such as chromatography-coupled high resolution tandem mass spectrometry, the stable isotope label can be precisely localized and quantified within the biomolecules. The application of stable isotope labelling to lipidomics is however complicated by the diversity of lipids and the complexity of the necessary data analysis. This article discusses key experimental aspects of stable isotope labelling in the field of mass spectrometry-based lipidomics, summarizes current applications and provides an outlook on future developments and potential.
在过去的二十年中，脂质的作用已不仅仅局限于细胞膜的组成部分和能量储存形式，现在还被认为在各种疾病中发挥着重要作用，脂质组学技术在研究和临床环境中的脂质生物标志物研究是应用最广泛的技术之一。稳定同位素标记已成为小分子代谢和动态分析的常用技术，因为它是唯一可以直接测量生物分子的生物合成、重塑和降解的实验设置。通过使用色谱-高分辨率串联质谱联用等最先进的分析技术，可以在生物分子内精确地定位和定量稳定同位素标记。然而，由于脂质的多样性和必要数据分析的复杂性，稳定同位素标记在脂质组学中的应用变得复杂。本文讨论了基于质谱的脂质组学领域中稳定同位素标记的关键实验方面，总结了当前的应用，并对未来的发展和潜力进行了展望。
