Berardi V P, Weeks K E, Steere A C
Department of Virology, Center for Disease Control, Boston, Massachusetts 02130.
J Infect Dis. 1988 Oct;158(4):754-60. doi: 10.1093/infdis/158.4.754.
We used an antibody-capture enzyme immunoassay (EIA) to evaluate the early antibody responses to Borrelia burgdorferi in paired sera from 30 patients with erythema chronicum migrans. During acute disease, 20 (67%) patients had elevated specific IgM responses, and by convalescence (one to four weeks after treatment), 28 (93%) patients had increased IgM or IgG responses. In acute specimens, elevated IgM responses correlated with disseminated infection; however, by convalescence, most patients with either localized or disseminated disease had positive tests. Among 133 control subjects, IgM cross-reactivity was observed in 4 of 37 patients with either Epstein-Barr virus or rickettsial infections, and false-positive IgG tests were seen in 8 of 28 patients with syphilis. With antibody-capture EIA, the diagnosis of Lyme disease can be confirmed in the majority of acutely ill patients and in almost all patients by convalescence.
我们采用抗体捕获酶免疫测定法（EIA），对30例慢性游走性红斑患者配对血清中针对伯氏疏螺旋体的早期抗体反应进行评估。在急性期，20例（67%）患者特异性IgM反应升高，到恢复期（治疗后1至4周），28例（93%）患者IgM或IgG反应增强。在急性期标本中，IgM反应升高与播散性感染相关；然而，到恢复期，大多数局限性或播散性疾病患者检测结果呈阳性。在133名对照受试者中，37例患有爱泼斯坦-巴尔病毒或立克次体感染的患者中有4例观察到IgM交叉反应，28例梅毒患者中有8例出现假阳性IgG检测结果。采用抗体捕获EIA法，大多数急性期患者以及几乎所有恢复期患者均可确诊莱姆病。
