Hébert G A, Cooksey R C, Clark N C, Hill B C, Jarvis W R, Thornsberry C
Hospital Infections Program, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1988 Oct;26(10):1950-6. doi: 10.1128/jcm.26.10.1950-1956.1988.
The biochemical profiles obtained with Staph-Ident (Analytab Products, Plainview, N.Y.) panels were combined with the results of adherence and synergistic hemolysis tests to define biotypes among 1,064 clinical isolates representing eight species of coagulase-negative staphylococci. The 672 isolates of Staphylococcus epidermidis were aligned in 69 of 144 potential biotypes in our scheme because of 18 different biochemical profiles and the eight physiologic subtypes. Isolates of most other species were in fewer biotypes because of more uniform adherence and synergistic hemolysis data, as well as fewer biochemical profiles. Since adherence and synergistic hemolysis may prove to be related to virulence and pathogenicity, biotyping with these test results would help evaluate the reliability of adherence and synergistic hemolysis as possible indices of the clinical significance of some of these organisms. When the antimicrobial susceptibility and plasmid profiles obtained on two clusters of S. epidermidis isolates were compared with the biotyping results, one cluster was not further differentiated by plasmid profiles, but was by antimicrobial profiles; the other cluster with only two biotypes was further divided into five distinct types by plasmid profiles but was not separated at all by antimicrobial profiles.
用葡萄球菌鉴定试剂盒（Analytab Products公司，纽约州普莱恩维尤）检测得到的生化特征，与黏附及协同溶血试验结果相结合，以确定1064株临床分离株的生物型，这些分离株代表了8种凝固酶阴性葡萄球菌。由于有18种不同的生化特征及8种生理亚型，在我们的方案中，672株表皮葡萄球菌分离株归入144种潜在生物型中的69种。大多数其他菌种的分离株由于黏附及协同溶血数据更为一致，且生化特征较少，因而归入的生物型也较少。由于黏附及协同溶血可能与毒力和致病性相关，利用这些试验结果进行生物分型将有助于评估黏附及协同溶血作为这些微生物某些临床意义可能指标的可靠性。当比较两群表皮葡萄球菌分离株的药敏谱和质粒谱与生物分型结果时，一群分离株不能通过质粒谱进一步区分，但可通过药敏谱区分；另一群只有两种生物型的分离株，通过质粒谱进一步分为5个不同类型，但完全不能通过药敏谱分开。
