南非采集的污水和贻贝中首次检测到 Aichi 病毒 1。
The First Molecular Detection of Aichi Virus 1 in Raw Sewage and Mussels Collected in South Africa.
机构信息
Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, 6140, South Africa.
Centre for Biomolecular Sciences, School of Biology, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews, Scotland, KY16 9ST, UK.
出版信息
Food Environ Virol. 2019 Mar;11(1):96-100. doi: 10.1007/s12560-018-9362-4. Epub 2018 Dec 17.
Aichi virus 1 (AiV-1) has a worldwide distribution and is associated with gastroenteritis in humans. In this study, raw sewage and mussel samples were analyzed for the presence of AiV-1 using reverse transcription-PCR (RT-PCR). Amplification and sequencing of the 3CD and VP1 genomic regions followed by phylogenetic analysis using selected genome sequences revealed the presence of AiV-1, genotype B. The results highlight the importance of further screening to evaluate the prevalence and epidemiology of this clinically important virus in South Africa.
甲型肝炎病毒 1 型（AiV-1）在全球范围内分布，并与人类的肠胃炎有关。在这项研究中，采用逆转录-PCR（RT-PCR）方法对原始污水和贻贝样本进行了 AiV-1 的检测。对 3CD 和 VP1 基因组区域进行扩增和测序，然后使用选定的基因组序列进行系统发育分析，显示存在甲型肝炎病毒 1 型，基因型 B。研究结果强调了进一步筛查的重要性，以评估这种具有临床重要性的病毒在南非的流行情况和流行病学。
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