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与不同抗生素联合使用对……的影响

Effect of in Combination With Different Antibiotics on .

作者信息

Yang Jingpeng, Yang Hong

机构信息

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

出版信息

Front Microbiol. 2018 Dec 4;9:2953. doi: 10.3389/fmicb.2018.02953. eCollection 2018.

DOI:10.3389/fmicb.2018.02953
PMID:30564210
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6288195/
Abstract

While combinations of probiotics with antibiotics have exhibited beneficial and adverse effects in the treatment of infection (CDI), no substantive explanation has been provided for these effects. In this study, ATCC 9689 (CD) was treated with (YH68) in combination with five different antibiotics to explore the effects of the different combinations on . Cell-free culture supernatant (CFCS) of YH68 was combined with metronidazole (MTR), vancomycin (VAN), clindamycin (CLI), ceftazidime (CAZ) or ampicillin (AMP) to treat CD. The plate counting method was used to determine the growth and spore production of CD, and cell damage was assessed by the measurement of extracellular ATP levels with a luminescence-based kit. The production of toxin A/B was measured with an ELISA kit. The gene expression levels of and in CD were evaluated by real-time qPCR. The CFCS of YH68 (3 × 10 CFU/mL) at 0.25 times the minimal inhibitory concentration (MIC) (0.25YH68) in combination with the five antibiotics exerted stronger inhibitory effects on the growth and spore production of CD than the same antibiotics in the absence of 0.25YH68, except 0.25YH68&MTR&AMP, 0.25YH68&MTR&CAZ, and 0.25YH68&VAN&CLI. However, treatment with 0.25YH68&VAN, 0.25YH68&AMP, 0.25YH68&MTR&CAZ, 0.25YH68&VAN&CAZ, 0.25YH68&VAN&AMP, and 0.25YH68&CAZ&AMP resulted in increased cell damage. In addition, the different combinations, except 0.25YH68&CLI, 0.25YH68&MTR&AMP and 0.25YH68&VAN&CLI, dramatically reduced the production of toxin A/B in comparison with the effects of the same antibiotics in the absence of 0.25YH68. The gene expression levels of and in CD were lowered upon treatment with 0.25YH68 in combination with MTR, CLI, CAZ, MTR&CAZ, MTR&AMP, CLI&CAZ, and CLI&AMP, whereas the levels were enhanced by 0.25YH68 in combination with VAN, AMP, MTR&CLI, VAN&CLI, VAN&AMP, and CAZ&AMP. In summary, YH68 in combination with specific antibiotics could enhance the inhibitory effects of antibiotics against CD. In addition, the antagonistic effects between some antibiotics could be weakened by YH68.

摘要

虽然益生菌与抗生素联合使用在艰难梭菌感染（CDI）治疗中已显示出有益和不良影响，但尚未对这些影响给出实质性解释。在本研究中，将ATCC 9689（艰难梭菌）用YH68与五种不同抗生素联合处理，以探究不同组合对艰难梭菌的影响。将YH68的无细胞培养上清液（CFCS）与甲硝唑（MTR）、万古霉素（VAN）、克林霉素（CLI）、头孢他啶（CAZ）或氨苄西林（AMP）联合用于处理艰难梭菌。采用平板计数法测定艰难梭菌的生长和芽孢产生情况，并使用基于发光的试剂盒通过测量细胞外ATP水平评估细胞损伤。用ELISA试剂盒测量毒素A/B的产生。通过实时定量PCR评估艰难梭菌中tcdA和tcdB的基因表达水平。YH68的CFCS（3×10 CFU/mL）在最小抑菌浓度（MIC）的0.25倍（0.25YH68）下与五种抗生素联合使用，对艰难梭菌的生长和芽孢产生的抑制作用比相同抗生素在无0.25YH68时更强，但0.25YH68&MTR&AMP、0.25YH68&MTR&CAZ和0.25YH68&VAN&CLI除外。然而，用0.25YH68&VAN、0.25YH68&AMP、0.25YH68&MTR&CAZ、0.25YH68&VAN&CAZ、0.25YH68&VAN&AMP和0.25YH68&CAZ&AMP处理会导致细胞损伤增加。此外，除0.25YH68&CLI、0.25YH68&MTR&AMP和0.25YH68&VAN&CLI外，与无0.25YH68时相同抗生素的作用相比，不同组合显著降低了毒素A/B的产生。用0.25YH68与MTR、CLI、CAZ、MTR&CAZ、MTR&AMP、CLI&CAZ和CLI&AMP联合处理后，艰难梭菌中tcdA和tcdB的基因表达水平降低，而用0.25YH

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f97e/6288195/994c5247af57/fmicb-09-02953-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f97e/6288195/e8c45942420a/fmicb-09-02953-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f97e/6288195/55f4ef5c8ed5/fmicb-09-02953-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f97e/6288195/994c5247af57/fmicb-09-02953-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f97e/6288195/e8c45942420a/fmicb-09-02953-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f97e/6288195/047ef33849a6/fmicb-09-02953-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f97e/6288195/3eb06f4dd0e7/fmicb-09-02953-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f97e/6288195/55f4ef5c8ed5/fmicb-09-02953-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f97e/6288195/994c5247af57/fmicb-09-02953-g005.jpg

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