Bioeffects Division, Air Force Research Laboratory, JBSA Fort Sam Houston, Texas.
Bioeffects Division, Air Force Research Laboratory, JBSA Fort Sam Houston, Texas.
Biophys J. 2019 Jan 8;116(1):120-126. doi: 10.1016/j.bpj.2018.11.3129. Epub 2018 Dec 1.
Direct observation of rapid membrane potential changes is critical to understand how complex neurological systems function. This knowledge is especially important when stimulation is achieved through an external stimulus meant to mimic a naturally occurring process. To enable exploration of this dynamic space, we developed an all-optical method for observing rapid changes in membrane potential at temporal resolutions of ∼25 ns. By applying a single 600-ns electric pulse, we observed sub-microsecond, continuous membrane charging and discharging dynamics. Close agreement between the acquired results and an analytical membrane-charging model validates the utility of this technique. This tool will deepen our understanding of the role of membrane potential dynamics in the regulation of many biological and chemical processes within living systems.
直接观察快速的膜电位变化对于理解复杂的神经系统功能至关重要。当刺激是通过外部刺激来模拟自然发生的过程时，这种知识尤为重要。为了能够探索这个动态空间，我们开发了一种全光学方法，用于以约 25ns 的时间分辨率观察膜电位的快速变化。通过施加单个 600ns 的电脉冲，我们观察到亚微秒的连续膜充电和放电动力学。获得的结果与有效的分析膜充电模型之间的紧密一致验证了该技术的实用性。该工具将加深我们对膜电位动力学在调节活体内许多生物和化学过程中的作用的理解。
