Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, Brisbane, QLD 4072, Australia.
Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, Brisbane, QLD 4072, Australia.
Virology. 2019 Feb;528:89-100. doi: 10.1016/j.virol.2018.12.006. Epub 2018 Dec 22.
The endosymbiotic bacterium Wolbachia pipientis has been shown to restrict a range of RNA viruses in Drosophila melanogaster and transinfected dengue mosquito, Aedes aegypti. Here, we show that Wolbachia infection enhances replication of Aedes albopictus densovirus (AalDNV-1), a single stranded DNA virus, in Aedes cell lines in a density-dependent manner. Analysis of previously produced small RNAs of Aag2 cells showed that Wolbachia-infected cells produced greater absolute abundance of virus-derived short interfering RNAs compared to uninfected cells. Additionally, we found production of virus-derived PIWI-like RNAs (vpiRNA) produced in response to AalDNV-1 infection. Nuclear fractions of Aag2 cells produced a primary vpiRNA signature U bias whereas the typical "ping-pong" signature (U - A) was evident in vpiRNAs from the cytoplasmic fractions. This is the first report of the density-dependent enhancement of DNA viruses by Wolbachia. Further, we report the generation of vpiRNAs in a DNA virus-host interaction for the first time.
共生菌沃尔巴克氏体已被证明可以限制黑腹果蝇和转染的登革热蚊子埃及伊蚊中的一系列 RNA 病毒。在这里，我们表明沃尔巴克氏体感染以密度依赖的方式增强了黄热病白纹伊蚊浓核病毒（AalDNV-1）的复制，黄热病白纹伊蚊浓核病毒是一种单链 DNA 病毒。对先前产生的 Aag2 细胞的小 RNA 进行分析表明，与未感染的细胞相比，感染沃尔巴克氏体的细胞产生了更大的病毒衍生短干扰 RNA 的绝对丰度。此外，我们发现 AalDNV-1 感染会产生病毒衍生的 PIWI 样 RNA (vpiRNA)。Aag2 细胞的核部分产生了主要的 vpiRNA U 偏向性，而细胞质部分的典型“乒乓”签名 (U - A) 在 vpiRNA 中是明显的。这是首次报道沃尔巴克氏体对 DNA 病毒的密度依赖性增强。此外，我们首次报道了 DNA 病毒-宿主相互作用中 vpiRNA 的产生。
