Scaringi L, Marconi P, Boccanera M, Tissi L, Bistoni F, Cassone A
Institute of Medical Microbiology, University of Perugia, Italy.
J Gen Microbiol. 1988 May;134(5):1265-74. doi: 10.1099/00221287-134-5-1265.
Cell wall components from Candida albicans were compared to intact cells for their ability to induce natural cytotoxic immunoeffectors in the peritoneal cavity of mice. A soluble mannoprotein extract (MP) and an insoluble glucan fraction (GG) strongly stimulated the generation of peritoneal effectors capable of lysing YAC-1 and P-815 tumour cell lines in vitro. The anti-YAC-1 effectors were characterized as natural killer (NK) lymphocytes while the anti-P-815 effectors appeared to be activated macrophages. The activity of each fraction was typically dose-dependent and both fractions differed from whole cells in the kinetics of induction of cytotoxicity. However, the NK and macrophage effectors generated by these materials had similar functional and phenotypic properties, irrespective of the material used as inducer. No mannoprotein was detected in the insoluble glucan fraction GG. Hence, the immunoenhancing activity of GG could not be attributed to the presence of some MP or MP-like component. Mannan-rich fractions with low (less than 3%) protein content (M) or extracted by hot alkaline reagent (M-alk) were inactive as NK and macrophage inducers. Thus, the cell wall of C. albicans contains at least two distinct macromolecular complexes which mediate the induction in murine peritoneal exudates of cytotoxic effectors active against tumour cell lines.
将白色念珠菌的细胞壁成分与完整细胞进行比较，以研究它们在小鼠腹腔中诱导天然细胞毒性免疫效应细胞的能力。一种可溶性甘露糖蛋白提取物（MP）和一种不溶性葡聚糖组分（GG）强烈刺激了能够在体外裂解YAC-1和P-815肿瘤细胞系的腹腔效应细胞的生成。抗YAC-1效应细胞被鉴定为自然杀伤（NK）淋巴细胞，而抗P-815效应细胞似乎是活化的巨噬细胞。每个组分的活性通常呈剂量依赖性，并且两个组分在细胞毒性诱导动力学方面与完整细胞不同。然而，这些物质产生的NK和巨噬细胞效应细胞具有相似的功能和表型特性，无论用作诱导剂的物质是什么。在不溶性葡聚糖组分GG中未检测到甘露糖蛋白。因此，GG 的免疫增强活性不能归因于某些MP或MP样成分的存在。蛋白质含量低（低于3%）的富含甘露聚糖的组分（M）或通过热碱性试剂提取的组分（M-alk）作为NK和巨噬细胞诱导剂无活性。因此，白色念珠菌的细胞壁含有至少两种不同的大分子复合物，它们介导在小鼠腹腔渗出物中诱导对肿瘤细胞系具有活性的细胞毒性效应细胞。
