Department of Medical Microbiology, St. Olavs Hospital, Trondheim University Hospital, Norway.
Department of Medical Microbiology, St. Olavs Hospital, Trondheim University Hospital, Norway; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Norway.
J Clin Virol. 2019 Feb;111:19-23. doi: 10.1016/j.jcv.2018.12.005. Epub 2018 Dec 18.
Human adenovirus (HAdV) is a double-stranded DNA virus associated with respiratory tract infections (RTI) in children. Using polymerase chain reaction (PCR) tests, HAdV often is detected together with other virus species, even in healthy controls.
The aim of this study was to compare molecular detection of HAdV with culture, and to examine the associations of various methods to RTI.
Nasopharyngeal aspirates (NPA) were collected from 4319 children admitted with RTI and from 361 controls. The NPAs were examined for 23 viral and bacterial pathogens, using inhouse real-time PCR-assays based on TaqMan probes, in addition to bacterial and viral culture. HAdV concentration was evaluated semi-quantitatively from the Ct-value and quantitatively by use of ADENOVIRUS R-gene®.
HAdV-DNA was detected in 6.1% patient samples and in 10.5% controls (p< 0.001). Compared to controls, patients had an OR of 3.8 (95% CI 1.4-10.3) for mono-detection of HAdV DNA, and an OR of 5.1 (95% CI 2.0-13.4) for HAdV-positive samples grew adenovirus by culture. HAdV DNA loads from children with RTI consisted of two clusters: one cluster with high viral loads (Ct < 30 and >106 copies/ml) and one cluster with low viral loads, whereas among the controls, nearly all had low viral loads (OR 7.8, 95% CI 2.2-27.1). In 61 available plasma samples, 16.4% were positive for HAdV DNA, all were from patients.
The detection of HAdV DNA per se by qualitative PCR is not useful as a diagnostic test. Detection of HAdV by use of viral culture and a high viral HAdV DNA load are the two methods most strongly associated with RTI in children.
人类腺病毒（HAdV）是一种双链 DNA 病毒，与儿童呼吸道感染（RTI）有关。使用聚合酶链反应（PCR）检测时，HAdV 经常与其他病毒一起被检测到，甚至在健康对照组中也是如此。
本研究旨在比较 HAdV 的分子检测与培养，并研究各种方法与 RTI 的关联。
从因 RTI 住院的 4319 名儿童和 361 名对照中采集鼻咽抽吸物（NPA）。使用 TaqMan 探针的基于 TaqMan 探针的内部实时 PCR 检测，以及细菌和病毒培养，对 23 种病毒和细菌病原体进行检测。通过使用 ADENOVIRUS R-gene®，根据 Ct 值对 HAdV 浓度进行半定量评估，并进行定量评估。
HAdV-DNA 在 6.1%的患者样本和 10.5%的对照中被检测到（p<0.001）。与对照组相比，HAdV DNA 单一检测的患者的 OR 为 3.8（95%CI 1.4-10.3），HAdV 阳性样本培养出腺病毒的 OR 为 5.1（95%CI 2.0-13.4）。患有 RTI 的儿童的 HAdV DNA 负荷由两个簇组成：一个高病毒负荷（Ct<30 和>106 拷贝/ml）的簇和一个低病毒负荷的簇，而对照组中，几乎所有的 HAdV DNA 负荷都较低（OR 7.8，95%CI 2.2-27.1）。在 61 份可获得的血浆样本中，有 16.4%的 HAdV DNA 呈阳性，均来自患者。
定性 PCR 本身检测 HAdV DNA 作为诊断测试没有用处。通过病毒培养和高病毒 HAdV DNA 负荷检测到 HAdV 是与儿童 RTI 最密切相关的两种方法。
