Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
BMC Pediatr. 2022 Oct 8;22(1):583. doi: 10.1186/s12887-022-03625-3.
Human adenovirus (HAdV) is an important viral agent in children which can lead to severe acute respiratory infection (SARI). Reports on molecular epidemiology of HAdVs in Iran are limited. This case-control study is conducted to compare the HAdV infection rate and molecular epidemiology among two groups of children with and without respiratory symptoms in Tehran, Iran during 2018-2019.
Nested PCR was performed on 120 oropharyngeal swabs taken from children aged five and younger with SARI who were hospitalized as the case group, and 120 oropharyngeal swabs were collected from children of the same age without respiratory symptoms as the control group. For positive samples Sanger sequencing was done and a phylogenetic tree was drawn afterward.
Out of 120 cases, 8 (6.6%) tested positive for eachHAdV types including 6 (75%) HAdV-B7, 1 (12.5%) HAdV-C2, and 1 (12.5%) HAdV-C6. Among the control group, out of 120 samples, 8 (6.6%) were positive comprising 5 (62.5%) HAdV-C5, 2 (25%) HAdV-F41, and 1 (12.5%) HAdV-C6.
The present study indicated a different viewpoint of HAdV molecular epidemiology in which the genotypes were compared in children with and without respiratory symptoms. HAdV prevalence was equally common in cases and controls but different genotypes were detected in these two groups. HAdV-B7 was the main type among children with SARI, dissimilar to children with no respiratory symptoms where HAdV-C5 was the predominant type. Detecting HAdV-F in oropharyngeal swabs was a rare finding, which requires further investigation.
人类腺病毒（HAdV）是导致儿童严重急性呼吸道感染（SARI）的重要病毒。关于伊朗 HAdV 分子流行病学的报告有限。本病例对照研究旨在比较 2018-2019 年伊朗德黑兰两组有和无呼吸道症状儿童的 HAdV 感染率和分子流行病学。
对 120 例年龄在 5 岁以下患有 SARI 的住院患儿（病例组）和 120 例年龄相同无呼吸道症状的患儿（对照组）的咽拭子进行巢式 PCR。对阳性样本进行 Sanger 测序，然后绘制系统发育树。
在 120 例病例中，每种 HAdV 类型的检测阳性率为 8（6.6%），包括 6（75%）HAdV-B7、1（12.5%）HAdV-C2 和 1（12.5%）HAdV-C6。在对照组中，120 个样本中有 8（6.6%）呈阳性，包括 5（62.5%）HAdV-C5、2（25%）HAdV-F41 和 1（12.5%）HAdV-C6。
本研究表明，HAdV 分子流行病学的观点不同，比较了有和无呼吸道症状的儿童的基因型。病例组和对照组的 HAdV 患病率相同，但这两组检测到的基因型不同。HAdV-B7 是 SARI 患儿的主要类型，与无呼吸道症状的患儿不同，HAdV-C5 是主要类型。在咽拭子中检测到 HAdV-F 是一种罕见的发现，需要进一步研究。
