Cieplik Fabian, Zaura Egija, Brandt Bernd W, Buijs Mark J, Buchalla Wolfgang, Crielaard Wim, Laine Marja L, Deng Dong Mei, Exterkate Rob A M
Department of Conservative Dentistry and Periodontology, University Medical Center Regensburg, Regensburg, Germany.
Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
J Oral Microbiol. 2018 Nov 27;11(1):1551596. doi: 10.1080/20022727.2018.1551596. eCollection 2019.
: Periodontal diseases are triggered by dysbiotic microbial biofilms. Therefore, it is essential to develop appropriate biofilm models. Aim of the present study was to culture microcosm biofilms inoculated from different niches in periodontitis patients and compare their microbial composition to those inoculated from subgingival plaque. : Saliva, subgingival plaque, tongue and tonsils were sampled in five periodontitis patients to serve as inocula for culturing biofilms in an active attachment model. Biofilms were grown for 14 or 28 d and analyzed for their microbial composition by 16S rDNA sequencing. : As classified by HOMD, all biofilms were dominated by periodontitis-associated taxa, irrespective which niche had been used for inoculation. There was a low similarity between 14 d biofilms and their respective inocula (Bray-Curtis similarity 0.26), while biofilms cultured for 14 and 28 d shared high similarity (0.69). Principal components analysis showed much stronger clustering per patient than per niche indicating that the choice of patients may be more crucial than choice of the respective niches in these patients. : Saliva, tongue scrapings or tonsil swabs may represent sufficient alternative inocula for growing microcosm biofilms resembling periodontitis-associated microbial communities in cases when sampling subgingival plaque is not possible.
牙周疾病由生态失调的微生物生物膜引发。因此，开发合适的生物膜模型至关重要。本研究的目的是培养取自牙周炎患者不同部位的微观生物膜，并将其微生物组成与取自龈下菌斑的生物膜进行比较。：对五名牙周炎患者的唾液、龈下菌斑、舌和扁桃体进行采样，作为在活性附着模型中培养生物膜的接种物。生物膜培养14天或28天，并通过16S rDNA测序分析其微生物组成。：根据人类口腔微生物数据库（HOMD）分类，所有生物膜均以与牙周炎相关的分类群为主，无论接种物取自哪个部位。14天生物膜与其各自接种物之间的相似性较低（Bray-Curtis相似性为0.26），而培养14天和28天的生物膜具有较高的相似性（0.69）。主成分分析显示，每位患者的聚类比每个部位更强，这表明在这些患者中，患者的选择可能比各自部位的选择更为关键。：在无法采集龈下菌斑的情况下，唾液、舌刮屑或扁桃体拭子可能是培养类似于牙周炎相关微生物群落的微观生物膜的足够替代接种物。
