Theoretical Soft Matter and Biophysics, Institute of Complex Systems and Institute for Advanced Simulation, Forschungszentrum Jülich, Jülich, Germany.
Elife. 2019 Jan 2;8:e39694. doi: 10.7554/eLife.39694.
We introduce a filament-based simulation model for coarse-grained, effective motor-mediated interaction between microtubule pairs to study the time-scales that compose cytoplasmic streaming. We characterise microtubule dynamics in two-dimensional systems by chronologically arranging five distinct processes of varying duration that make up streaming, from microtubule pairs to collective dynamics. The structures found were polarity sorted due to the propulsion of antialigned microtubules. This also gave rise to the formation of large polar-aligned domains, and streaming at the domain boundaries. Correlation functions, mean squared displacements, and velocity distributions reveal a cascade of processes ultimately leading to microtubule streaming and advection, spanning multiple microtubule lengths. The characteristic times for the processes extend over three orders of magnitude from fast single-microtubule processes to slow collective processes. Our approach can be used to directly test the importance of molecular components, such as motors and crosslinking proteins between microtubules, on the collective dynamics at cellular scale.
我们引入了一种基于纤维的模拟模型，用于粗粒度的、有效的、由马达介导的微管对之间的相互作用，以研究构成细胞质流动的时间尺度。我们通过按时间顺序排列构成流动的五个不同的、持续时间不同的过程，来描述二维系统中的微管动力学，从微管对到集体动力学。由于不对齐微管的推进，发现的结构被极性排序。这也导致了大的极性对齐域的形成，以及在域边界的流动。相关函数、均方位移和速度分布揭示了一个级联过程，最终导致微管流动和输运，跨越多个微管长度。过程的特征时间跨越三个数量级，从快速的单微管过程到缓慢的集体过程。我们的方法可用于直接测试分子成分（如马达和微管之间的交联蛋白）对细胞尺度上集体动力学的重要性。
