古菌门中存在光感应、卟啉生物合成、卡尔文-本森-巴斯汉姆循环和尿素生产的基因组和转录组证据。

Genomic and transcriptomic evidence of light-sensing, porphyrin biosynthesis, Calvin-Benson-Bassham cycle, and urea production in Bathyarchaeota.

机构信息

Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen, China.

Laboratory of Environmental Microbiology and Toxicology, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, China.

出版信息

Microbiome. 2020 Mar 31;8(1):43. doi: 10.1186/s40168-020-00820-1.

BACKGROUND

Bathyarchaeota, a newly proposed archaeal phylum, is considered as an important driver of the global carbon cycle. However, due to the great diversity of them, there is limited genomic information that accurately encompasses the metabolic potential of the entire archaeal phylum.

RESULTS

In the current study, nine metagenome-assembled genomes of Bathyarchaeota from four subgroups were constructed from mangrove sediments, and metatranscriptomes were obtained for evaluating their in situ transcriptional activities. Comparative analyses with reference genomes and the transcripts of functional genes posit an expanded role for Bathyarchaeota in phototrophy, autotrophy, and nitrogen and sulfur cycles, respectively. Notably, the presence of genes for rhodopsins, cobalamin biosynthesis, and the oxygen-dependent metabolic pathways in some Bathyarchaeota subgroup 6 genomes suggest a light-sensing and microoxic lifestyle within this subgroup.

CONCLUSIONS

The results of this study expand our knowledge of metabolic abilities and diverse lifestyles of Bathyarchaeota, highlighting the crucial role of Bathyarchaeota in geochemical cycle. Video abstract.

背景

深海古菌是一个新提出的古菌门，被认为是全球碳循环的重要驱动者。然而，由于它们的多样性很大，因此只有有限的基因组信息能够准确涵盖整个古菌门的代谢潜力。

结果

在本研究中，从红树林沉积物中构建了来自四个亚群的九个深海古菌门的宏基因组组装基因组，并获得了用于评估其原位转录活性的宏转录组。与参考基因组和功能基因转录本的比较分析表明，深海古菌在光合作用、自养、氮和硫循环中分别具有扩展的作用。值得注意的是，一些深海古菌亚群 6 基因组中存在视黄醛、钴胺素生物合成和需氧代谢途径的基因，这表明该亚群中存在光感应和微氧生活方式。