Microbiology Department, Faculty of Pharmacy, Misr International University, Cairo 19648, Egypt.
Pharmaceutics and Industrial Pharmacy Department, Faculty of Pharmacy, Ain Shams University, African Union Organization St., Abbassia, Cairo 11566, Egypt.
Biomed Res Int. 2018 Nov 28;2018:7658238. doi: 10.1155/2018/7658238. eCollection 2018.
Antibiotic-resistant and biofilm-forming bacteria have surprisingly increased over recent years. On the contrary, the rate of development of new antibiotics to treat these emerging superbugs is very slow. Therefore, the aim of this study was to prepare novel nanobiotic formulations to improve the antimicrobial activity of three antibiotics (linezolid, doxycycline, and clindamycin) against . Antibiotics were formulated as nanoemulsions and evaluated for their antimicrobial activities and cytotoxicities. Cytotoxicity of the conventional antibiotics and nanobiotics was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on rat hepatocytes. Half-maximal inhibitory concentration (IC) was estimated from an experimentally derived dose-response curve for each concentration using GraphPad Prism software. Upon quantitative assessment of biofilm formation, eighty-four isolates (66.14 %) were biofilm forming. Linezolid and doxycycline nanobiotics exhibited promising antibacterial activities. On the contrary, clindamycin nanobiotic exhibited poor antibacterial activity. Minimum biofilm inhibitory concentrations showed that 73.68 %, 45.6%, and 5.2% of isolates were sensitive to linezolid, doxycycline, and clindamycin nanobiotics, respectively. Results of this study revealed that antibiotics loaded in nanosystems had a higher antimicrobial activity and lower cytotoxicities as compared to those of conventional free antibiotics, indicating their potential therapeutic values.
近年来，抗生素耐药和形成生物膜的细菌数量惊人地增加。相比之下，用于治疗这些新兴超级细菌的新抗生素的开发速度非常缓慢。因此，本研究的目的是制备新型纳米抗生素制剂，以提高三种抗生素（利奈唑胺、强力霉素和克林霉素）对 的抗菌活性。将抗生素制成纳米乳剂，并评估其抗菌活性和细胞毒性。使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法在大鼠肝细胞上分析常规抗生素和纳米抗生素的细胞毒性。使用 GraphPad Prism 软件从每个浓度的实验衍生剂量-反应曲线中估算半最大抑制浓度 (IC)。在对 生物膜形成进行定量评估后，发现 84 株分离物（66.14%）为生物膜形成。利奈唑胺和强力霉素纳米抗生素表现出有希望的抗菌活性。相比之下，克林霉素纳米抗生素表现出较差的抗菌活性。最低生物膜抑制浓度显示，73.68%、45.6%和 5.2%的分离物分别对利奈唑胺、强力霉素和克林霉素纳米抗生素敏感。这项研究的结果表明，与常规游离抗生素相比，负载在纳米系统中的抗生素具有更高的抗菌活性和更低的细胞毒性，表明它们具有潜在的治疗价值。
