大肠杆菌硒代半胱氨酸tRNA异常长的氨基酸接受茎是由核糖核酸酶P的异常切割导致的。
The unusually long amino acid acceptor stem of Escherichia coli selenocysteine tRNA results from abnormal cleavage by RNase P.
作者信息
Burkard U, Söll D
机构信息
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
出版信息
Nucleic Acids Res. 1988 Dec 23;16(24):11617-24. doi: 10.1093/nar/16.24.11617.
The nucleotide sequence of the gene encoding the Escherichia coli selenocysteine tRNA (tRNA(SeCys] predicts an unusually long acceptor stem of 8 base pairs (one more than other tRNAs). Here we show by in vivo experiments (Northern blots, primer extension analysis) and by in vitro RNA processing studies that E. coli tRNA(SeCys) does contain this additional basepair, and that its formation results from abnormal cleavage by RNase P.
编码大肠杆菌硒代半胱氨酸tRNA（tRNA[SeCys]）的基因的核苷酸序列预测其接受茎异常长，有8个碱基对（比其他tRNA多一个）。在这里，我们通过体内实验（Northern印迹、引物延伸分析）和体外RNA加工研究表明，大肠杆菌tRNA(SeCys)确实含有这个额外的碱基对，并且它的形成是由RNase P的异常切割导致的。
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