Lee Jin Hwan, Lee Hyeon-Jin, Choung Myoung-Gun
Department of Monitoring and Analysis, NAKDONG River Basin Environmental Office, Ministry of Environment, Changwon 641-722, Republic of Korea.
Department of Herbal Medicine Resource, Dogye Campus, Kangwon National University, Hwangjori # 3, Dogye-up, Samcheok 245-907, Republic of Korea.
Food Chem. 2011 Nov 15;129(2):272-278. doi: 10.1016/j.foodchem.2011.04.040. Epub 2011 Apr 21.
The objectives of the present study were to investigate anthocyanin profiles and their biological properties, including antioxidant, anticancer, and antiallergic, from the red petals of Korean edible rose (Rosa hybrida cv. Noblered). The acidic methanol extract of this species showed potent biological activities at a concentration of 50μg/mL. Its anthocyanins were characterised as cyanidin 3,5-di-O-glucoside and pelargonidin 3,5-di-O-glucoside using reversed-phase C column chromatography, NMR spectroscopy, and HPLC-DAD-ESI/MS analysis. Cyanidin 3,5-di-O-glucoside was the predominant constituent (375mg/100g), representing about 85% of total content. Cyanidin 3,5-di-O-glucoside exhibited good scavenging activity against DPPH radical with IC value of 55.2μg/mL; pelargonidin 3,5-di-O-glucoside showed potent anticancer effects against LNCap (human prostate cell line), ACHN (human renal cell line) and MOLT-4F (human leukaemia cell line) cell cultures, with IC values of 6.43, 18.3, and 6.78μg/mL, respectively. Antiallergic activities were only moderate.
本研究的目的是调查韩国食用玫瑰（Rosa hybrida cv. Noblered）红色花瓣中的花色苷谱及其生物学特性，包括抗氧化、抗癌和抗过敏特性。该物种的酸性甲醇提取物在浓度为50μg/mL时表现出强大的生物活性。使用反相C柱色谱、核磁共振光谱和高效液相色谱-二极管阵列-电喷雾电离/质谱分析，其花色苷被鉴定为矢车菊素3,5-二-O-葡萄糖苷和天竺葵素3,5-二-O-葡萄糖苷。矢车菊素3,5-二-O-葡萄糖苷是主要成分（375mg/100g），约占总含量的85%。矢车菊素3,5-二-O-葡萄糖苷对DPPH自由基表现出良好的清除活性，IC值为55.2μg/mL；天竺葵素3,5-二-O-葡萄糖苷对LNCap（人前列腺癌细胞系）、ACHN（人肾癌细胞系）和MOLT-4F（人白血病细胞系）细胞培养物显示出强大的抗癌作用，IC值分别为6.43、18.3和6.78μg/mL。抗过敏活性仅为中等。
