Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, USA.
Scripps Center for Oceans and Human Health, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, USA.
Environ Microbiol. 2019 May;21(5):1575-1585. doi: 10.1111/1462-2920.14532. Epub 2019 Feb 22.
The production of pentabromopseudilin and related brominated compounds by Pseudoalteromonas spp. has recently been linked to the bmp biosynthetic gene cluster. This study explored the distribution and evolutionary history of this gene cluster in the genus Pseudoalteromonas. A phylogeny of the genus revealed numerous clades that do not contain type strains, suggesting considerable species level diversity has yet to be described. Comparative genomics revealed four distinct versions of the gene cluster distributed among 19 of the 101 Pseudoalteromonas genomes examined. These were largely localized to the least inclusive clades containing the Pseudoalteromonas luteoviolacea and Pseudoalteromonas phenolica type strains and show clear evidence of gene and gene cluster loss in certain lineages. Bmp gene phylogeny is largely congruent with the Pseudoalteromonas species phylogeny, suggesting vertical inheritance within the genus. However, the gene cluster is found in three different genomic environments suggesting either chromosomal rearrangement or multiple acquisition events. Bmp conservation within certain lineages suggests the encoded products are highly relevant to the ecology of these bacteria.
近年来，假交替单胞菌属（Pseudoalteromonas spp.）产生的五溴基别隐醇和相关溴化化合物与 bmp 生物合成基因簇有关。本研究探讨了该基因簇在假交替单胞菌属中的分布和进化历史。属的系统发育揭示了许多不包含模式株的分支，这表明仍有相当多的物种水平多样性有待描述。比较基因组学揭示了在 101 个假交替单胞菌基因组中检查的 19 个基因组中存在四个不同版本的基因簇。这些基因簇主要定位于包含假交替单胞菌黄紫质（Pseudoalteromonas luteoviolacea）和假交替单胞菌酚（Pseudoalteromonas phenolica）模式株的最小包容性分支，并且在某些谱系中显示出明显的基因和基因簇丢失的证据。Bmp 基因系统发育与假交替单胞菌的物种系统发育基本一致，表明属内的垂直遗传。然而，该基因簇存在于三种不同的基因组环境中，这表明可能发生了染色体重排或多次获得事件。Bmp 在某些谱系中的保守性表明编码产物与这些细菌的生态学高度相关。
