Rattanakreetakul Chainarong, Keawmanee Pisut, Bincader Santiti, Mongkolporn Orarat, Phuntumart Vipaporn, Chiba Sotaro, Pongpisutta Ratiya
Department of Plant Pathology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom 73140, Thailand.
Program Plant Science, Agricultural Technology and Agro-Industry Faculty, Rajamangala University of Technology Suvarnabhumi, Phra Nakhon Si Ayutthaya 13000, Thailand.
Plants (Basel). 2023 Mar 2;12(5):1130. doi: 10.3390/plants12051130.
Anthracnose caused by spp. is one of the major problems in mango production worldwide, including Thailand. All mango cultivars are susceptible, but Nam Dok Mai See Thong (NDMST) is the most vulnerable. Through a single spore isolation method, a total of 37 isolates of spp. were obtained from NDMST showing anthracnose symptoms. Identification was performed using a combination of morphology characteristics, Koch's postulates, and phylogenetic analysis. The pathogenicity assay and Koch's postulates on leaves and fruit confirmed that all spp. tested were causal agents of mango anthracnose. Multilocus analysis using DNA sequences of internal transcribed spacer (ITS) regions, β-tubulin (), actin (), and chitin synthase () was performed for molecular identification. Two concatenated phylogenetic trees were constructed using either two-loci of ITS and , or four-loci of ITS, , , and Both phylogenetic trees were indistinguishable and showed that these 37 isolates belong to , , , and Our results indicated that using at least two loci of ITS and were sufficient to infer species complexes. Of 37 isolates, was the most dominant species (19 isolates), followed by (10 isolates), (5 isolates), and (3 isolates). In Thailand, and have been reported to cause anthracnose in mango, however, this is the first report of and associated with mango anthracnose in central Thailand.
由[病原菌名称未给出]引起的炭疽病是包括泰国在内的全球芒果生产中的主要问题之一。所有芒果品种都易感，但南多迈通（Nam Dok Mai See Thong，NDMST）最为脆弱。通过单孢分离法，从表现出炭疽病症状的NDMST中总共获得了37株[病原菌名称未给出]分离株。使用形态特征、柯赫氏法则和系统发育分析相结合的方法进行鉴定。对叶片和果实的致病性测定及柯赫氏法则证实，所有测试的[病原菌名称未给出]都是芒果炭疽病的病原菌。利用内部转录间隔区（ITS）区域、β-微管蛋白（[具体基因未给出]）、肌动蛋白（[具体基因未给出]）和几丁质合成酶（[具体基因未给出]）的DNA序列进行多位点分析以进行分子鉴定。使用ITS和[具体基因未给出]的两个位点，或ITS、[具体基因未给出]、[具体基因未给出]和[具体基因未给出]的四个位点构建了两个串联系统发育树。两个系统发育树无法区分，表明这37株分离株属于[具体菌属未给出]、[具体菌属未给出]、[具体菌属未给出]和[具体菌属未给出]。我们的结果表明，使用至少ITS和[具体基因未给出]的两个位点足以推断[病原菌名称未给出]的物种复合体。在37株分离株中，[具体菌属未给出]是最主要的物种（19株），其次是[具体菌属未给出]（10株）、[具体菌属未给出]（5株）和[具体菌属未给出]（3株）。在泰国，[具体菌属未给出]和[具体菌属未给出]已被报道可引起芒果炭疽病，然而，这是泰国中部与芒果炭疽病相关的[具体菌属未给出]和[具体菌属未给出]的首次报道。
