Two Newly Identified Species Associated with Mango Anthracnose in Central Thailand.

Anthracnose caused by spp. is one of the major problems in mango production worldwide, including Thailand. All mango cultivars are susceptible, but Nam Dok Mai See Thong (NDMST) is the most vulnerable. Through a single spore isolation method, a total of 37 isolates of spp. were obtained from NDMST showing anthracnose symptoms. Identification was performed using a combination of morphology characteristics, Koch's postulates, and phylogenetic analysis. The pathogenicity assay and Koch's postulates on leaves and fruit confirmed that all spp. tested were causal agents of mango anthracnose. Multilocus analysis using DNA sequences of internal transcribed spacer (ITS) regions, β-tubulin (), actin (), and chitin synthase () was performed for molecular identification. Two concatenated phylogenetic trees were constructed using either two-loci of ITS and , or four-loci of ITS, , , and Both phylogenetic trees were indistinguishable and showed that these 37 isolates belong to , , , and Our results indicated that using at least two loci of ITS and were sufficient to infer species complexes. Of 37 isolates, was the most dominant species (19 isolates), followed by (10 isolates), (5 isolates), and (3 isolates). In Thailand, and have been reported to cause anthracnose in mango, however, this is the first report of and associated with mango anthracnose in central Thailand.