The Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu, 610065, China.
School of Light Industry and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Shandong, 250353, China.
Environ Sci Pollut Res Int. 2019 Mar;26(7):7277-7283. doi: 10.1007/s11356-019-04226-x. Epub 2019 Jan 25.
In this work, we investigate collagen protein powder (CPP) extracted from chromium leather scrap waste (CLSW). The composition and molecular weight distribution of CPP were determined by elemental analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. The microstructure and size distribution of CPP were then characterized by scanning electron microscopy (SEM) and nanometer analyzer instrument. Finally, CPP was treated with corn starch (CS), and the swelling behavior of the resulting CPP-CS blend was investigated in order to determine its range of applications. The experimental data showed that CPP contains 13 different amino-acids. CPP also displayed low mineral salt levels and a nitrogen content of 43.84%, indicating its potential use as an organic fertilizer. The molecular weight range of CPP is 6.5 to ~ 26.6 kDa. After the obtained CPP was blended with CS, the CPP-CS blend is endowed with optimal swelling properties and is able to overcome the solubility drawbacks of CPP alone. In addition, when the CPP was used as a natural fertilizer, the germination rate and height of kidney beans obviously increased.
本工作研究了从铬革渣废料（CLSW）中提取的胶原蛋白蛋白粉（CPP）。通过元素分析和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分别确定 CPP 的组成和分子量分布。然后通过扫描电子显微镜（SEM）和纳米粒度分析仪对 CPP 的微观结构和粒径分布进行了表征。最后，用玉米淀粉（CS）对 CPP 进行处理，研究了所得 CPP-CS 共混物的溶胀行为，以确定其应用范围。实验数据表明 CPP 含有 13 种不同的氨基酸。CPP 还显示出低矿物质盐含量和 43.84%的氮含量，表明其可能用作有机肥料。CPP 的分子量范围为 6.5 至~26.6 kDa。获得的 CPP 与 CS 混合后，CPP-CS 共混物具有最佳的溶胀性能，可以克服 CPP 单独存在的溶解度缺陷。此外，当 CPP 用作天然肥料时，明显提高了芸豆的发芽率和高度。
