Department of Endodontics, School of Dentistry, Grande Rio University (UNIGRANRIO), Rio de Janeiro, Brazil.
Department of Endodontics, Rio de Janeiro State University (UERJ), Rio de Janeiro, Brazil.
Int Endod J. 2019 Jul;52(7):1063-1069. doi: 10.1111/iej.13085. Epub 2019 Feb 20.
To investigate the reliability of using bovine teeth as an alternative to human teeth in an intra-tooth push-out model by comparing the bond strength of three root canal sealers in both tooth substrates.
From human and bovine incisors (12 per group), one 1-mm disc was obtained from each root. On each disc, three standard 0.8-mm-diameter holes were drilled through the dentine. After irrigation, the holes were filled with one of three root canal sealers: AH Plus, MTA Fillapex or Total Fill BC Sealer. Tooth slices were kept in contact with sterile gauze moistened in PBS solution (pH = 7.2) for 7 days at 37 °C, and finally, a push-out load was applied until sealer dislocation. The bonded interface area was calculated to obtain data in MPa. The push-out data for each sealer and the overall push-out data for human and bovine teeth were compared (Mann-Whitney U). Kruskal-Wallis was used to study the sealers behaviour at each of the dental substrates. Multiple comparisons were performed using Mann-Whitney U after Bonferroni correction (P < 0.05).
Dentine substrate did not influence the push-out of either individual sealers or overall sealers tested (Mann-Whitney U, P > 0.05). In both human and bovine samples, AH Plus performed better than BC Sealer and MTA Fillapex, whilst BC Sealer also provided a superior push-out compared to MTA Fillapex (Mann-Whitney U, P < 0.05).
When using an intra-tooth model, bovine teeth did not significantly influence the bond strength of sealers.
通过比较三种根管封闭剂在两种牙本质基底中的粘结强度，研究将牛牙用作牙内推出模型中替代人牙的可靠性。
从人及牛切牙（每组 12 个）中，每个牙根获得 1 个 1mm 圆盘。在每个圆盘上，通过牙本质钻三个标准的 0.8mm 直径的孔。冲洗后，用三种根管封闭剂中的一种填充孔：AH Plus、MTA Fillapex 或 Total Fill BC 密封剂。牙切片用无菌纱布保持接触，纱布用 PBS 溶液（pH=7.2）湿润，在 37°C 下放置 7 天，最后施加推出负荷，直至密封剂脱位。计算粘结界面面积以获得 MPa 数据。比较每种密封剂的推出数据以及人牙和牛牙的总体推出数据（Mann-Whitney U）。Kruskal-Wallis 用于研究每个牙本质基底上密封剂的行为。使用 Bonferroni 校正后，通过 Mann-Whitney U 进行多重比较（P<0.05）。
牙本质基底不影响单独测试的密封剂或总体密封剂的推出（Mann-Whitney U，P>0.05）。在人牙和牛牙样本中，AH Plus 的推出性能优于 BC 密封剂和 MTA Fillapex，而 BC 密封剂的推出性能也优于 MTA Fillapex（Mann-Whitney U，P<0.05）。
在使用牙内模型时，牛牙对密封剂的粘结强度没有显著影响。
