School of Medical Instruments and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
College of Information Engineering, Shanghai Maritime University, Shanghai 200135, China.
Molecules. 2019 Jan 29;24(3):483. doi: 10.3390/molecules24030483.
Lévl. et Vant essential oil could be used as a good antimicrobial flavouring agent and applied in the food industry. In this study, three methods, including simultaneous distillation-extraction (SDE), subcritical extraction and hydrodistillation, were applied to extract essential oil. Compared with subcritical extraction (1%) and hydrodistillation (0.5%), SDE gave a higher yield (1.2%). Components of the essential oils were analysed with gas chromatography-mass spectrometry (GC-MS), and the most abundant ingredients were caryophyllene oxide, neointermedeol, borneol, α-thujone and β-caryophyllene. These five components accounted for 82.93%, 40.90% and 40.33% for SDE, subcritical extraction, and hydrodistillation, respectively. Based on agar disc diffusion and minimum inhibitory concentration (MIC) assays, SDE oil showed a significant inhibitory effect towards , , , and . Furthermore, electron microscope observations (SEM) confirmed that SDE oil could obviously deform cell morphology and destroy the structure of cell walls. Performances showed that SDE was a promising process for extracting essential oil with both high yield and antimicrobial activity.
莱弗氏和万特尔精油可用作良好的抗菌调味剂，并应用于食品工业。本研究采用同时蒸馏萃取（SDE）、亚临界萃取和水蒸馏法提取精油。与亚临界萃取（1%）和水蒸馏（0.5%）相比，SDE 法的收率更高（1.2%）。采用气相色谱-质谱联用仪（GC-MS）分析精油成分，含量最丰富的成分是石竹烯氧化物、新蛇床内酯、龙脑、α-侧柏酮和β-石竹烯。这 5 种成分在 SDE、亚临界萃取和水蒸馏中的含量分别为 82.93%、40.90%和 40.33%。基于琼脂圆盘扩散和最小抑菌浓度（MIC）测定，SDE 油对 、 、 、 和 表现出显著的抑制作用。此外，电子显微镜观察（SEM）证实 SDE 油可明显改变细胞形态，破坏细胞壁结构。结果表明，SDE 是一种很有前途的提取精油的方法，具有产率高和抗菌活性强的特点。
