Department of Chemistry, Pusan National University, Busan 46241, South Korea.
Biomedical System & Technology Group, Korea Institute of Industrial Technology, Cheonan 31056, South Korea.
Biosens Bioelectron. 2019 Apr 1;130:48-54. doi: 10.1016/j.bios.2019.01.028. Epub 2019 Jan 21.
Enzymatic and non-enzymatic amperometric glucose sensors based on nanostructured Au-Ni alloy were prepared and compared in their performance. The hierarchically structured Au-Ni surface was merely used for the non-enzymatic glucose sensor, while glucose oxidase attached poly-3'(benzoic acid) -2,2':5',2'- terthiophene (pTBA) formed on the alloy surface was used as the enzymatic sensor. The fabricated sensor was characterized using surface analysis and electrochemical experiments. In case of the enzymatic sensor, the anodic current of HO generated from the enzyme reaction was used as the analytical signal, while the direct oxidation of glucose was observed on a mere Au-Ni alloy electrode without enzyme immobilization, which shows an excellent catalytic oxidation of glucose even in physiological pH. The potential pulse pretreatment of the sensor surfaces improved the performance, which allowed both the sensors reproducible and reusable (enzymatic sensor: coefficient of variation = 1.82%, n = 5, non-enzymatic: coefficient of variation = 2.93%). The enzymatic biosensor reveals the advantages of increased sensitivity, selectivity, and stability, compared with the non-enzymatic sensor. The linear range of enzymatic sensor was attained from 1.0 µM to 30.0 mM with a detection limit of 0.29 μM. The reliabilities of the sensors were also demonstrated through the glucose analysis in human blood samples, and the result was compared with the commercially available glucometer.
基于纳米结构 Au-Ni 合金的酶和非酶安培葡萄糖传感器的制备及其性能进行了比较。分层结构的 Au-Ni 表面仅用于非酶葡萄糖传感器，而附着在合金表面上的葡萄糖氧化酶接枝聚 3'（苯甲酸）-2,2':5',2'-三联噻吩（pTBA）则用于酶传感器。所制备的传感器采用表面分析和电化学实验进行了表征。在酶传感器的情况下，将酶反应生成的 HO 的阳极电流用作分析信号，而在没有酶固定化的情况下，仅仅在 Au-Ni 合金电极上就观察到了葡萄糖的直接氧化，这表明即使在生理 pH 值下，葡萄糖也具有出色的催化氧化性能。传感器表面的电位脉冲预处理提高了性能，使两种传感器都具有可重现性和可重复使用性（酶传感器：变异系数=1.82%，n=5，非酶：变异系数=2.93%）。与非酶传感器相比，酶生物传感器具有灵敏度、选择性和稳定性提高的优势。酶传感器的线性范围从 1.0µM 到 30.0mM，检测限为 0.29µM。通过在人血样中进行葡萄糖分析也证明了传感器的可靠性，并将结果与市售血糖仪进行了比较。
