Lindner Marco, Tresztenyak Aliz, Fülöp Gergö, Jahr Wiebke, Prinz Adrian, Prinz Iris, Danzl Johann G, Schütz Gerhard J, Sevcsik Eva
Institute of Applied Physics, TU Wien, Vienna, Austria.
Stratec Consumables GmbH, Anif, Austria.
Front Chem. 2019 Jan 24;6:655. doi: 10.3389/fchem.2018.00655. eCollection 2018.
Protein micropatterning has become an important tool for many biomedical applications as well as in academic research. Current techniques that allow to reduce the feature size of patterns below 1 μm are, however, often costly and require sophisticated equipment. We present here a straightforward and convenient method to generate highly condensed nanopatterns of proteins without the need for clean room facilities or expensive equipment. Our approach is based on nanocontact printing and allows for the fabrication of protein patterns with feature sizes of 80 nm and periodicities down to 140 nm. This was made possible by the use of the material X-poly(dimethylsiloxane) (X-PDMS) in a two-layer stamp layout for protein printing. In a proof of principle, different proteins at various scales were printed and the pattern quality was evaluated by atomic force microscopy (AFM) and super-resolution fluorescence microscopy.
蛋白质微图案化已成为许多生物医学应用以及学术研究中的重要工具。然而，目前能够将图案特征尺寸减小到1μm以下的技术通常成本高昂，并且需要精密的设备。我们在此提出一种简单便捷的方法，无需洁净室设施或昂贵设备即可生成高度浓缩的蛋白质纳米图案。我们的方法基于纳米接触印刷，能够制造特征尺寸为80nm且周期低至140nm的蛋白质图案。这是通过在用于蛋白质印刷的双层印章布局中使用X-聚二甲基硅氧烷（X-PDMS）材料实现的。在原理验证中，印刷了不同尺度的不同蛋白质，并通过原子力显微镜（AFM）和超分辨率荧光显微镜评估了图案质量。
