AgNPs 和 AuNPs 诱导的薰衣草体外培养物精油的气相色谱-质谱（GC-MS）分析。
Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Essential Oils from AgNPs and AuNPs Elicited Lavandula angustifolia in vitro Cultures.
机构信息
Faculty of Chemical Technology and Engineering, Department of Organic and Physical Chemistry, WestPomeranian University of Technology in Szczecin, Aleja Piastów 42, 71-065 Szczecin, Poland.
Department of Plant Genetics, Breeding and Biotechnology, Faculty of Environmental Management andAgriculture, West Pomeranian University of Technology in Szczecin, ul. Słowackiego 17, 71-434 Szczecin,Poland.
出版信息
Molecules. 2019 Feb 9;24(3):606. doi: 10.3390/molecules24030606.
The aim of this study was to determine how the addition of gold and silver nanoparticles to culture media affects the composition of essential oils extracted from Lavandula angustifolia propagated on MS media with the addition of 10 and 50 mg•dm-3 of gold (24.2 ± 2.4 nm) and silver (27.5 ± 4.8 nm) nanocolloids. The oil extracted from the lavender tissues propagated on the medium with 10 mg•dm-3 AgNPs (silver nanoparticles) differed the most with respect to the control; oil-10 compounds were not found at all, and 13 others were detected which were not present in the control oil. The addition of AuNPs (gold nanoparticles) and AgNPs to the media resulted in a decrease of lower molecular weight compounds (e.g., α- and β-pinene, camphene, δ-3-carene, p-cymene, 1,8-cineole, trans-pinocarveol, camphoriborneol), which were replaced by those of a higher molecular weight (τ- and α-cadinol 9-cedranone, cadalene, α-bisabolol, cis-14-nor-muurol-5-en-4-one, (E,E)-farnesol).
本研究旨在确定在添加 10 和 50 mg·dm-3 金（24.2 ± 2.4nm）和银纳米胶体（27.5 ± 4.8nm）的 MS 培养基中培养的薰衣草组织中添加金和银纳米粒子对薰衣草精油成分的影响。在添加 10 mg·dm-3 AgNPs（银纳米粒子）的培养基中培养的薰衣草组织中提取的精油与对照品差异最大；对照品中根本没有发现油-10 化合物，而检测到了其他 13 种在对照油中不存在的化合物。向培养基中添加 AuNPs（金纳米粒子）和 AgNPs 导致低分子量化合物（例如α-和β-蒎烯、莰烯、δ-3-蒈烯、对伞花烃、1,8-桉树脑、反式-松油醇、龙脑烯醛）减少，而高分子量化合物（τ-和α-杜松醇、9-雪松酮、雪松烯、α-毕澄茄醇、顺-14-去甲-5-愈创木烯-4-酮、（E，E）-法呢醇）增加。
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