Thermo Fisher Scientific, 2 Radcliff Rd., Tewksbury, MA 01876, USA.
Biosensors (Basel). 2019 Feb 12;9(1):25. doi: 10.3390/bios9010025.
Raman microspectroscopy is now well established as one of the most powerful analytical techniques for a diverse range of applications in physical (material) and biological sciences. Consequently, the technique provides exceptional analytical opportunities to the science and technology of biosensing due to its capability to analyze both parts of a biosensor system-biologically sensitive components, and a variety of materials and systems used in physicochemical transducers. Recent technological developments in Raman spectral imaging have brought additional possibilities in two- and three-dimensional (2D and 3D) characterization of the biosensor's constituents and their changes on a submicrometer scale in a label-free, real-time nondestructive method of detection. In this report, the essential components and features of a modern confocal Raman microscope are reviewed using the instance of Thermo Scientific DXRxi Raman imaging microscope, and examples of the potential applications of Raman microscopy and imaging for constituents of biosensors are presented.
拉曼微光谱分析现已成为物理（材料）和生物科学中各种应用最强大的分析技术之一。因此，由于其能够分析生物传感器系统的两个部分——生物敏感组件以及用于物理化学换能器的各种材料和系统，该技术为生物传感的科学和技术提供了特殊的分析机会。拉曼光谱成像的最新技术发展为生物传感器成分的二维和三维（2D 和 3D）特征化以及在无标记、实时无损检测方法中以亚微米级分辨率检测它们的变化带来了额外的可能性。在本报告中，使用 Thermo Scientific DXRxi 拉曼成像显微镜的实例，回顾了现代共焦拉曼显微镜的基本组成部分和特点，并展示了拉曼显微镜和成像在生物传感器成分方面的潜在应用示例。
