Nashine Sonali, Kanodia Raj, Nesburn Anthony B, Soman Girish, Kuppermann Baruch D, Kenney M Cristina
Department of Ophthalmology, Gavin Herbert Eye Institute, University of California Irvine, Irvine, CA 92697, USA.
Rhinoplasty Surgeon, Dr. Raj Kanodia Medical Group, Beverly Hills, CA 90210, USA.
Aging (Albany NY). 2019 Feb 21;11(4):1177-1188. doi: 10.18632/aging.101820.
Gaetrn i.e., Indian gooseberry/ Amla) (EO) has been used extensively as a nutraceutical in several diseases since it is known to boost immunity and offers numerous health benefits such as antioxidant, anti-inflammatory, and anti-aging effects. The goal of our study was to test the hypothesis that EO will rescue human AMD RPE transmitochondrial cells from mitochondria-induced cellular damage. AMD RPE transmitochondrial cell lines were created by fusion of mitochondria DNA-deficient APRE-19 () cells with platelets isolated from AMD patients, and therefore had identical nuclei but differed in mitochondrial DNA content. These AMD RPE cells were treated with EO extract followed by characterization of effects of EO using cellular and molecular assays. Herein, EO significantly improved live cell number and mitochondrial membrane potential, reduced apoptosis and oxidative stress, down-regulated , and up-regulated . In conclusion, EO improved cellular and mitochondrial health, thereby playing a key cytoprotective role in AMD . Further studies are required to examine the mechanisms that mediate the cytoprotective effects of EO.
余甘子（即印度醋栗/阿育吠陀）精油（EO）已被广泛用作多种疾病的营养保健品，因为它已知能增强免疫力，并提供许多健康益处，如抗氧化、抗炎和抗衰老作用。我们研究的目的是检验以下假设：EO将拯救人类年龄相关性黄斑变性（AMD）视网膜色素上皮（RPE）转线粒体细胞免受线粒体诱导的细胞损伤。AMD RPE转线粒体细胞系是通过线粒体DNA缺陷的APRE-19细胞与从AMD患者分离的血小板融合而创建的，因此具有相同的细胞核，但线粒体DNA含量不同。这些AMD RPE细胞用EO提取物处理，然后使用细胞和分子分析方法对EO的作用进行表征。在此，EO显著提高了活细胞数量和线粒体膜电位，减少了细胞凋亡和氧化应激，下调了……，并上调了……。总之，EO改善了细胞和线粒体健康，从而在AMD中发挥关键的细胞保护作用。需要进一步研究以探讨介导EO细胞保护作用的机制。
