从深海来源的 sp. SCSIO ZS0098 中鉴定出抗微生物 Aborycin 生物合成基因簇，使其能够在异源宿主中生产。
Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived sp. SCSIO ZS0098 Enables Production in a Heterologous Host.
机构信息
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 110039, China.
出版信息
Mar Drugs. 2019 Feb 21;17(2):127. doi: 10.3390/md17020127.
Aborycin is a ribosomally synthesized member of the type I lasso peptide natural products. In the present study, aborycin was isolated and identified from the deep-sea-derived microbe sp. SCSIO ZS0098. The aborycin biosynthetic gene cluster () was identified on the basis of genome sequence analyses and then heterologously expressed in M1152 to effectively produce aborycin. Aborycin generated in this fashion exhibited moderate antibacterial activity against 13 strains from various sources with minimum inhibitory concentrations MICs = 8.0~128 µg/mL, against ATCC 29212 with an MIC = 8.0 µg/mL, and against with MIC = 2.0 µg/mL. Additionally, aborycin displayed potent antibacterial activity (MIC = 0.5 µg/mL) against the poultry pathogen 5F52C. The reported cluster clearly has the potential to provide a means of expanding the repertoire of anti-infective type I lasso peptides.
阿波霉素是一类核糖体合成的 I 型套索肽天然产物。本研究从深海来源的微生物 sp. SCSIO ZS0098 中分离并鉴定出阿波霉素。基于基因组序列分析，确定了阿波霉素生物合成基因簇（），并在 M1152 中异源表达，有效地生产出阿波霉素。这种方式产生的阿波霉素对来自不同来源的 13 株菌表现出中等的抗菌活性，最小抑菌浓度（MIC）为 8.0~128μg/mL，对 ATCC 29212 的 MIC 为 8.0μg/mL，对 的 MIC 为 2.0μg/mL。此外，阿波霉素对禽病原体 5F52C 表现出很强的抗菌活性（MIC = 0.5μg/mL）。所报道的 基因簇显然具有扩展抗感染 I 型套索肽 repertoire 的潜力。
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