Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX, USA.
Department of Biophysics, UT Southwestern Medical Center, Dallas, TX, USA.
Nat Methods. 2019 Mar;16(3):235-238. doi: 10.1038/s41592-019-0327-9. Epub 2019 Feb 25.
We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical design, higher light throughput and simultaneous multicolor illumination. Further, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral beam scanning.
我们介绍了一种场合成的方法和定理，可以通过对一维强度分布的非相干叠加，从任何扫描或抖动的光片（包括在晶格光片显微镜（LLSM）中使用的光片）中合成光片。与 LLSM 相比，这种用户友好且模块化的方法具有简化的光学设计、更高的光通量和同时多色照明的优点。此外，场合成比横向光束扫描产生的光片具有更低的光漂白率。
