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体内迁移细胞中液泡形成过程中的流体动力学。

Fluid dynamics during bleb formation in migrating cells in vivo.

机构信息

Institute of Cell Biology, ZMBE, Münster, Germany.

Institut Pasteur, Bioimage Analysis Unit, Paris, France.

出版信息

PLoS One. 2019 Feb 26;14(2):e0212699. doi: 10.1371/journal.pone.0212699. eCollection 2019.

DOI:10.1371/journal.pone.0212699
PMID:30807602
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6391022/
Abstract

Blebs are cellular protrusions observed in migrating cells and in cells undergoing spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm during bleb formation and the concurrent changes in cell volume using zebrafish primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation occurs concomitantly with cytoplasmic inflow into it and that during this process the total cell volume does not change. We thus show that bleb formation in primordial germ cells results primarily from redistribution of material within the cell rather than being driven by flow of water from an external source.

摘要

水泡是在迁移细胞和正在进行扩散、胞质分裂和细胞凋亡的细胞中观察到的细胞突起。在这里，我们以斑马鱼原始生殖细胞 (PGC) 作为体内模型，研究了水泡形成过程中的细胞质流动和同时发生的细胞体积变化。我们表明，水泡的膨胀伴随着细胞质的流入，在此过程中总细胞体积不变。因此，我们表明，原始生殖细胞中的水泡形成主要是由于细胞内物质的重新分配，而不是由外部来源的水流驱动的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e990/6391022/6d8daf813282/pone.0212699.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e990/6391022/5a31941973d0/pone.0212699.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e990/6391022/b2c11debecee/pone.0212699.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e990/6391022/6d8daf813282/pone.0212699.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e990/6391022/5a31941973d0/pone.0212699.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e990/6391022/b2c11debecee/pone.0212699.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e990/6391022/6d8daf813282/pone.0212699.g003.jpg

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本文引用的文献

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Dev Cell. 2017 Dec 4;43(5):577-587.e5. doi: 10.1016/j.devcel.2017.10.030. Epub 2017 Nov 22.
2
Plasma membrane changes during programmed cell deaths.程序性细胞死亡过程中的质膜变化。
Cell Res. 2018 Jan;28(1):9-21. doi: 10.1038/cr.2017.133. Epub 2017 Oct 27.
3
Guidelines for morpholino use in zebrafish.斑马鱼中吗啉代使用指南。
细胞表面过剩的变化与 3D 运动中的突起动力学相协调。
Biophys J. 2023 Sep 19;122(18):3656-3677. doi: 10.1016/j.bpj.2023.04.023. Epub 2023 May 18.
4
Blebs-Formation, Regulation, Positioning, and Role in Amoeboid Cell Migration.水泡的形成、调控、定位及其在变形细胞迁移中的作用
Front Cell Dev Biol. 2022 Jul 15;10:926394. doi: 10.3389/fcell.2022.926394. eCollection 2022.
5
Zebrafish Primordial Germ Cell Migration.斑马鱼原始生殖细胞迁移
Front Cell Dev Biol. 2021 Jun 23;9:684460. doi: 10.3389/fcell.2021.684460. eCollection 2021.
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Chemokine-biased robust self-organizing polarization of migrating cells in vivo.趋化因子偏向的体内迁移细胞的强健自组织极化。
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