Comparative sialotranscriptome analysis of the rare Chinese cicada Subpsaltria yangi, with identification of candidate genes related to host-plant adaptation.

Little is known about the mechanisms underlying the relationship between changes in salivary proteins and the adaptation of insects to different host-plants. To address this knowledge gap, the transcriptional profiles of salivary glands were compared among three populations of the rare cicada Subpsaltria yangi, in which two populations specialize on Zizyphus jujuba var. spinosa, but the population occurring in the Helan (HL) Mountains is locally specialized on the endemic plant Ephedra lepidosperma. The comparisons indicate that genes related to digestion and detoxification are differentially regulated in populations feeding on different plants, possibly reflecting adaptative changes in salivary proteins of S. yangi in response to different host chemistries. In detail, 38 differentially expressed genes and 21 up-regulated genes related to digestion and detoxification were identified respectively in two pairwise comparisons among the populations using different hosts, with some genes exclusively expressed in the HL population. Our results are consistent with the hypothesis that the host plant shift in the HL population was facilitated by differential regulation of genes related to digestion and detoxification. This study provides new information for elucidating the molecular mechanisms underlying the relationship between changed salivary proteins and the adaptability of plant-feeding insects to novel host plants.