Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, 78712, USA.
Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, 78712, USA.
Nat Commun. 2019 Feb 27;10(1):958. doi: 10.1038/s41467-019-08880-0.
The catalytic activity of the ribosome is mediated by RNA, yet proteins are essential for the function of the peptidyl transferase center (PTC). In eukaryotes, final assembly of the PTC occurs in the cytoplasm by insertion of the ribosomal protein Rpl10 (uL16). We determine structures of six intermediates in late nuclear and cytoplasmic maturation of the large subunit that reveal a tightly-choreographed sequence of protein and RNA rearrangements controlling the insertion of Rpl10. We also determine the structure of the biogenesis factor Yvh1 and show how it promotes assembly of the P stalk, a critical element for recruitment of GTPases that drive translation. Together, our structures provide a blueprint for final assembly of a functional ribosome.
核糖体的催化活性是由 RNA 介导的，但蛋白质对于肽基转移酶中心（PTC）的功能至关重要。在真核生物中，PTC 的最终组装是在细胞质中通过核糖体蛋白 Rpl10（uL16）的插入来完成的。我们确定了大亚基在核内和细胞质晚期成熟过程中的六个中间产物的结构，这些结构揭示了控制 Rpl10 插入的蛋白质和 RNA 重排的严格协调序列。我们还确定了生物发生因子 Yvh1 的结构，并展示了它如何促进 P 臂的组装，P 臂是招募驱动翻译的 GTPases 的关键元件。我们的结构共同为功能性核糖体的最终组装提供了蓝图。
