Department of Toxicology, Faculty of Pharmacy with Division of Laboratory Diagnostics, Wroclaw Medical University, Borowska Street 211, 50-556, Wrocław, Poland.
Department of Laboratory Diagnostics, Faculty of Pharmacy with Division of Laboratory Diagnostics, Wroclaw Medical University, Borowska Street 211a, 50-556, Wrocław, Poland.
Pathol Oncol Res. 2020 Apr;26(2):821-831. doi: 10.1007/s12253-019-00620-5. Epub 2019 Mar 4.
In recent years, bladder cancer (BC) has been reported as one of the most commonly occurring cancers among older people, and its detection is still difficult. Therefore, there is a need to search for additional useful markers of disease. Some studies indicate the important roles of inflammation and oxidative stress (OS) in bladder tumour pathogenesis. The aim of this study was to examine the levels of selected markers of OS, inflammation and angiogenesis in blood plasma/serum samples derived from patients with BC, and a healthy control group. Moreover the degrees of change and strength of correlation between values of the analysed markers and tumour stage or grade were estimated. Concentrations of: malondialdehyde (MDA) and advanced oxidation protein products (AOPP), and total antioxidant status (TAS) divided into slow (TAS-s) and fast (TAS-f) antioxidants (spectrophotometric measurement), angiogenin (ANG) (immunoenzymatic method) and C-reactive protein (CRP) (immunoturbidimetric method) were determined in both the studied groups. The majority of values of the examined parameters were significantly higher among patients, while subfractions of TAS were significantly lower in comparison to the control group. Moreover, different values and different strengths of correlation between the examined parameters and cancer stage or grade were noticed. The most significant changes for CRP were observed in T2 and for MDA in G3, while the lowest TAS-f activity was revealed in G1 patients. Increased values of OS parameters, angiogenesis and inflammation markers, in combination with reduced TAS subfractions activity in BC are important in its pathogenesis and will be helpful in estimation of patients' condition.
近年来，膀胱癌（BC）已被报道为老年人中最常见的癌症之一，但其检测仍然困难。因此，需要寻找其他有用的疾病标志物。一些研究表明炎症和氧化应激（OS）在膀胱癌发病机制中起着重要作用。本研究旨在检查来自 BC 患者和健康对照组的血浆/血清样本中 OS、炎症和血管生成的选定标志物的水平。此外，还估计了分析标志物的值与肿瘤分期或分级之间的变化程度和相关性强度。使用分光光度法测定丙二醛（MDA）和高级氧化蛋白产物（AOPP）以及总抗氧化状态（TAS）分为慢速（TAS-s）和快速（TAS-f）抗氧化剂（TAS-s）和快速（TAS-f）抗氧化剂（分光光度法），用免疫酶法测定血管生成素（ANG）和 C 反应蛋白（CRP）（免疫比浊法）。与对照组相比，研究组中大多数检查参数的值明显更高，而 TAS 的亚组分明显更低。此外，还注意到检查参数与癌症分期或分级之间的不同值和不同相关性强度。CRP 的变化最显著是在 T2 期，MDA 的变化最显著是在 G3 期，而 G1 期患者的 TAS-f 活性最低。BC 中 OS 参数、血管生成和炎症标志物的增加值，以及 TAS 亚组分活性的降低，在其发病机制中很重要，并有助于评估患者的病情。
