用于治疗骨关节炎的硫酸软骨素镁的合成。
Synthesis of chondroitin sulfate magnesium for osteoarthritis treatment.
机构信息
Department of Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China; Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, PR China.
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, PR China.
出版信息
Carbohydr Polym. 2019 May 15;212:387-394. doi: 10.1016/j.carbpol.2019.02.061. Epub 2019 Feb 18.
Magnesium chondroitin sulfate (MgCS) has been fabricated and characterized in this study. We investigated its morphology, composition as well as structure. The results verify that the sodium of sodium chondroitin sulfate (CS) is successfully replaced by magnesium and formed a polysaccharide-metal ion complex. To evaluate the clinical potential of MgCS, cell proliferation and apoptosis test were conducted. The results reveal that MgCS could effectively increase the proliferation and decrease the apoptosis of osteoarthritis (OA) chondrocytes. Moreover, real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to evaluate the gene expression level. RT-qPCR analysis suggests that MgCS could significantly increase the expression of COLII and decrease the expression of IL-1β and iNOS in OA chondrocytes. Furthermore, significant upregulation of Bcl-2 mRNA expression and downregulation of the expression of apoptosis related gene p53 were observed. Thus, it is indicated that MgCS should have great potentials in OA treatment.
本研究制备并表征了硫酸软骨素镁（MgCS）。我们研究了其形态、组成和结构。结果证实，硫酸软骨素（CS）的钠离子被镁成功取代，并形成了多糖-金属离子复合物。为了评估 MgCS 的临床潜力，进行了细胞增殖和凋亡试验。结果表明，MgCS 可有效增加骨关节炎（OA）软骨细胞的增殖，减少其凋亡。此外，还进行了实时定量聚合酶链反应（RT-qPCR）以评估基因表达水平。RT-qPCR 分析表明，MgCS 可显著增加 OA 软骨细胞中 COLII 的表达，并降低 IL-1β和 iNOS 的表达。此外，还观察到凋亡相关基因 p53 的表达下调和 Bcl-2 mRNA 表达上调。因此，表明 MgCS 在 OA 治疗中具有巨大潜力。
Zotero 插件