• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

深入了解 GRAS 转录因子在丛枝菌根共生中的复杂作用。

Insights into the complex role of GRAS transcription factors in the arbuscular mycorrhiza symbiosis.

机构信息

Unit IV-Plant Genomics, Institute of Plant Genetics, Leibniz Universität Hannover, Herrenhäuser Str. 2, D-30419, Hannover, Germany.

Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133, Milano, Italy.

出版信息

Sci Rep. 2019 Mar 4;9(1):3360. doi: 10.1038/s41598-019-40214-4.

DOI:10.1038/s41598-019-40214-4
PMID:30833646
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6399340/
Abstract

To improve access to limiting nutrients, the vast majority of land plants forms arbuscular mycorrhizal (AM) symbioses with Glomeromycota fungi. We show here that AM-related GRAS transcription factors from different subgroups are upregulated during a time course of mycorrhization. Based on expression studies in mutants defective in arbuscule branching (ram1-1, with a deleted MtRam1 GRAS transcription factor gene) or in the formation of functional arbuscules (pt4-2, mutated in the phosphate transporter gene MtPt4), we demonstrate that the five AM-related GRAS transcription factor genes MtGras1, MtGras4, MtGras6, MtGras7, and MtRad1 can be differentiated by their dependency on MtRAM1 and MtPT4, indicating that the network of AM-related GRAS transcription factors consists of at least two regulatory modules. One module involves the MtRAM1- and MtPT4-independent transcription factor MtGRAS4 that activates MtGras7. Another module is controlled by the MtRAM1- and MtPT4-dependent transcription factor MtGRAS1. Genome-wide expression profiles of mycorrhized MtGras1 knockdown and ram1-1 roots differ substantially, indicating different targets. Although an MtGras1 knockdown reduces transcription of AM-related GRAS transcription factor genes including MtRam1 and MtGras7, MtGras1 overexpression alone is not sufficient to activate MtGras genes. MtGras1 knockdown roots display normal fungal colonization, with a trend towards the formation of smaller arbuscules.

摘要

为了改善限制营养物质的获取，绝大多数陆地植物与 Glomeromycota 真菌形成丛枝菌根（AM）共生体。我们在这里展示，不同亚组的与 AM 相关的 GRAS 转录因子在共生体形成的时间过程中被上调。基于在分支菌（ram1-1，其 MtRam1 GRAS 转录因子基因缺失）或功能性菌根（pt4-2，磷酸盐转运蛋白基因 MtPt4 突变）缺陷突变体中的表达研究，我们证明了五个与 AM 相关的 GRAS 转录因子基因 MtGras1、MtGras4、MtGras6、MtGras7 和 MtRad1 可以通过它们对 MtRAM1 和 MtPT4 的依赖性来区分，表明与 AM 相关的 GRAS 转录因子网络至少由两个调节模块组成。一个模块涉及 MtRAM1 和 MtPT4 不依赖的转录因子 MtGras4，它激活 MtGras7。另一个模块由 MtRAM1 和 MtPT4 依赖的转录因子 MtGras1 控制。共生体 MtGras1 敲低和 ram1-1 根的全基因组表达谱有很大差异，表明有不同的靶点。尽管 MtGras1 敲低降低了包括 MtRam1 和 MtGras7 在内的 AM 相关 GRAS 转录因子基因的转录，但 MtGras1 过表达本身不足以激活 MtGras 基因。MtGras1 敲低根显示正常的真菌定殖，菌根变小的趋势。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/3915ca8c691b/41598_2019_40214_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/5c65664b4ee6/41598_2019_40214_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/90420c4f77a6/41598_2019_40214_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/091e2470714a/41598_2019_40214_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/aeb07858aa20/41598_2019_40214_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/b25fce18c301/41598_2019_40214_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/47b5874cc187/41598_2019_40214_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/0d3b35d35249/41598_2019_40214_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/3915ca8c691b/41598_2019_40214_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/5c65664b4ee6/41598_2019_40214_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/90420c4f77a6/41598_2019_40214_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/091e2470714a/41598_2019_40214_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/aeb07858aa20/41598_2019_40214_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/b25fce18c301/41598_2019_40214_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/47b5874cc187/41598_2019_40214_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/0d3b35d35249/41598_2019_40214_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f145/6399340/3915ca8c691b/41598_2019_40214_Fig8_HTML.jpg

相似文献

1
Insights into the complex role of GRAS transcription factors in the arbuscular mycorrhiza symbiosis.深入了解 GRAS 转录因子在丛枝菌根共生中的复杂作用。
Sci Rep. 2019 Mar 4;9(1):3360. doi: 10.1038/s41598-019-40214-4.
2
Medicago truncatula mtpt4 mutants reveal a role for nitrogen in the regulation of arbuscule degeneration in arbuscular mycorrhizal symbiosis.蒺藜苜蓿 mtpt4 突变体揭示了氮在丛枝菌根共生中泡囊退化调节中的作用。
Plant J. 2011 Dec;68(6):954-65. doi: 10.1111/j.1365-313X.2011.04746.x. Epub 2011 Oct 17.
3
Pre-announcement of symbiotic guests: transcriptional reprogramming by mycorrhizal lipochitooligosaccharides shows a strict co-dependency on the GRAS transcription factors NSP1 and RAM1.共生伙伴的预先宣告：菌根脂壳寡糖介导的转录重编程显示出对GRAS转录因子NSP1和RAM1的严格共同依赖性。
BMC Genomics. 2015 Nov 23;16:994. doi: 10.1186/s12864-015-2224-7.
4
A roadmap of cell-type specific gene expression during sequential stages of the arbuscular mycorrhiza symbiosis.丛枝菌根共生的连续阶段中细胞类型特异性基因表达的路线图。
BMC Genomics. 2013 May 7;14:306. doi: 10.1186/1471-2164-14-306.
5
Medicago truncatula and Glomus intraradices gene expression in cortical cells harboring arbuscules in the arbuscular mycorrhizal symbiosis.蒺藜苜蓿和根内球囊霉在丛枝菌根共生中含丛枝的皮层细胞中的基因表达。
BMC Plant Biol. 2009 Jan 22;9:10. doi: 10.1186/1471-2229-9-10.
6
The mycorrhiza-dependent defensin MtDefMd1 of Medicago truncatula acts during the late restructuring stages of arbuscule-containing cells.蒺藜苜蓿中依赖菌根的防御素MtDefMd1在含丛枝细胞的后期重组阶段发挥作用。
PLoS One. 2018 Jan 25;13(1):e0191841. doi: 10.1371/journal.pone.0191841. eCollection 2018.
7
Medicago AP2-Domain Transcription Factor WRI5a Is a Master Regulator of Lipid Biosynthesis and Transfer during Mycorrhizal Symbiosis.紫花苜蓿 AP2 结构域转录因子 WRI5a 是菌根共生中脂质生物合成和转移的主要调控因子。
Mol Plant. 2018 Nov 5;11(11):1344-1359. doi: 10.1016/j.molp.2018.09.006. Epub 2018 Oct 4.
8
Role of the GRAS transcription factor ATA/RAM1 in the transcriptional reprogramming of arbuscular mycorrhiza in Petunia hybrida.GRAS转录因子ATA/RAM1在矮牵牛丛枝菌根转录重编程中的作用。
BMC Genomics. 2017 Aug 8;18(1):589. doi: 10.1186/s12864-017-3988-8.
9
The Petunia GRAS Transcription Factor ATA/RAM1 Regulates Symbiotic Gene Expression and Fungal Morphogenesis in Arbuscular Mycorrhiza.矮牵牛GRAS转录因子ATA/RAM1调节丛枝菌根中的共生基因表达和真菌形态发生。
Plant Physiol. 2015 Jul;168(3):788-97. doi: 10.1104/pp.15.00310. Epub 2015 May 13.
10
Network of GRAS transcription factors involved in the control of arbuscule development in Lotus japonicus.参与控制日本百脉根丛枝发育的GRAS转录因子网络。
Plant Physiol. 2015 Mar;167(3):854-71. doi: 10.1104/pp.114.255430. Epub 2015 Jan 5.

引用本文的文献

1
Genome-Wide Identification, Expression, and Protein Interaction of Family Genes During Arbuscular Mycorrhizal Symbiosis in .丛枝菌根共生过程中 家族基因的全基因组鉴定、表达及蛋白质相互作用
Int J Mol Sci. 2025 Feb 27;26(5):2082. doi: 10.3390/ijms26052082.
2
Spatial co-transcriptomics reveals discrete stages of the arbuscular mycorrhizal symbiosis.空间共转录组学揭示了丛枝菌根共生的不同阶段。
Nat Plants. 2024 Apr;10(4):673-688. doi: 10.1038/s41477-024-01666-3. Epub 2024 Apr 8.
3
Molecular and Systems Biology Approaches for Harnessing the Symbiotic Interaction in Mycorrhizal Symbiosis for Grain and Oil Crop Cultivation.

本文引用的文献

1
Medicago AP2-Domain Transcription Factor WRI5a Is a Master Regulator of Lipid Biosynthesis and Transfer during Mycorrhizal Symbiosis.紫花苜蓿 AP2 结构域转录因子 WRI5a 是菌根共生中脂质生物合成和转移的主要调控因子。
Mol Plant. 2018 Nov 5;11(11):1344-1359. doi: 10.1016/j.molp.2018.09.006. Epub 2018 Oct 4.
2
AP2 transcription factor CBX1 with a specific function in symbiotic exchange of nutrients in mycorrhizal .AP2 转录因子 CBX1 在共生体中营养物质交换方面具有特定功能。
Proc Natl Acad Sci U S A. 2018 Sep 25;115(39):E9239-E9246. doi: 10.1073/pnas.1812275115. Epub 2018 Sep 12.
3
Transcriptional Regulation of Arbuscular Mycorrhiza Development.
利用菌根共生中共生相互作用的分子和系统生物学方法来进行谷物和油类作物种植。
Int J Mol Sci. 2024 Jan 11;25(2):912. doi: 10.3390/ijms25020912.
4
Network of GRAS transcription factors in plant development, fruit ripening and stress responses.植物发育、果实成熟和应激反应中GRAS转录因子网络
Hortic Res. 2023 Sep 27;10(12):uhad220. doi: 10.1093/hr/uhad220. eCollection 2023 Dec.
5
Analysis of the molecular and biochemical mechanisms involved in the symbiotic relationship between fungi and Crantz.对真菌与克兰茨之间共生关系所涉及的分子和生化机制的分析。
Front Plant Sci. 2023 Mar 7;14:1130924. doi: 10.3389/fpls.2023.1130924. eCollection 2023.
6
Nutrition vs association: plant defenses are altered by arbuscular mycorrhizal fungi association not by nutritional provisioning alone.营养与关联：植物防御因丛枝菌根真菌的关联而改变，而不仅仅是营养供应。
BMC Plant Biol. 2022 Aug 16;22(1):400. doi: 10.1186/s12870-022-03795-3.
7
Molecular Regulation of Arbuscular Mycorrhizal Symbiosis.丛枝菌根共生的分子调控。
Int J Mol Sci. 2022 May 25;23(11):5960. doi: 10.3390/ijms23115960.
8
Multifarious and Interactive Roles of GRAS Transcription Factors During Arbuscular Mycorrhiza Development.GRAS转录因子在丛枝菌根发育过程中的多种交互作用
Front Plant Sci. 2022 Mar 28;13:836213. doi: 10.3389/fpls.2022.836213. eCollection 2022.
丛枝菌根发育的转录调控。
Plant Cell Physiol. 2018 Apr 1;59(4):673-690. doi: 10.1093/pcp/pcy024.
4
UniProt: the universal protein knowledgebase.通用蛋白质知识库：UniProt
Nucleic Acids Res. 2018 Mar 16;46(5):2699. doi: 10.1093/nar/gky092.
5
The mycorrhiza-dependent defensin MtDefMd1 of Medicago truncatula acts during the late restructuring stages of arbuscule-containing cells.蒺藜苜蓿中依赖菌根的防御素MtDefMd1在含丛枝细胞的后期重组阶段发挥作用。
PLoS One. 2018 Jan 25;13(1):e0191841. doi: 10.1371/journal.pone.0191841. eCollection 2018.
6
The Medicago truncatula GRAS protein RAD1 supports arbuscular mycorrhiza symbiosis and Phytophthora palmivora susceptibility.蒺藜苜蓿 GRAS 蛋白 RAD1 支持丛枝菌根共生和菜豆疫霉侵染。
J Exp Bot. 2017 Dec 16;68(21-22):5871-5881. doi: 10.1093/jxb/erx398.
7
Genome-wide characterization of GRAS family genes in Medicago truncatula reveals their evolutionary dynamics and functional diversification.蒺藜苜蓿中GRAS家族基因的全基因组特征揭示了它们的进化动态和功能多样化。
PLoS One. 2017 Sep 25;12(9):e0185439. doi: 10.1371/journal.pone.0185439. eCollection 2017.
8
Understanding the Arbuscule at the Heart of Endomycorrhizal Symbioses in Plants.理解植物内生菌根共生体中心的丛枝菌根。
Curr Biol. 2017 Sep 11;27(17):R952-R963. doi: 10.1016/j.cub.2017.06.042.
9
Cell Biology: Control of Partner Lifetime in a Plant-Fungus Relationship.细胞生物学：植物与真菌关系中伙伴寿命的控制
Curr Biol. 2017 Jun 5;27(11):R420-R423. doi: 10.1016/j.cub.2017.04.020.
10
A Transcriptional Program for Arbuscule Degeneration during AM Symbiosis Is Regulated by MYB1.共生体中丛枝菌根退化的转录程序受 MYB1 调控。
Curr Biol. 2017 Apr 24;27(8):1206-1212. doi: 10.1016/j.cub.2017.03.003. Epub 2017 Apr 6.