miR-197-5p（一种心力衰竭的循环生物标志物）与C期或D期心力衰竭患者心肌纤维化及不良心血管事件的关联

Association of miR-197-5p, a Circulating Biomarker for Heart Failure, with Myocardial Fibrosis and Adverse Cardiovascular Events among Patients with Stage C or D Heart Failure.

作者信息

Liu Wei, Zheng Junmeng, Dong Jianzeng, Bai Rong, Song Deli, Ma Xiaohai, Zhao Lei, Yao Yan, Zhang Haibo, Liu Tong

机构信息

Department of Cardiology, Beijing AnZhen Hospital, Capital Medical University, Beijing, China.

Department of Cardiothoracic Surgery, Second Affiliated Hospital of Sun Yat-Sen University, Guang Dong, China.

出版信息

Cardiology. 2018;141(4):212-225. doi: 10.1159/000493419. Epub 2019 Mar 5.

DOI:10.1159/000493419

PMID:30836355

Abstract

OBJECTIVE

The aim of this study was to identify heart failure (HF)-specific circulating micro-RNAs (miRNA), and examine whether the selected miRNAs correlate with myocardial fibrosis and are reflective of the incidence of adverse cardiovascular events in patients with stage C or D HF.

METHODS

Circulating miRNAs which were expressed in end-stage HF patients and matched healthy controls were detected by microarray analysis and validated by quantitative real-time polymerase chain reaction. Multivariate Cox regression analysis was performed to determine whether the selected circulating miRNAs could be prognostic factors in HF patients.

RESULTS

In a cohort of 7 healthy controls and 9 patients with stage C or D HF, 7 miRNAs were differentially expressed. These miRNAs were further investigated in a second cohort of 80 patients with stage C or D HF and 30 healthy controls. Only miR-197-5P correlated with fibrosis as seen in cardiac magnetic resonance imaging in patients under the age of 50 years with stage C or D HF (r = 0.42, p = 0.008). Multivariate analyses revealed that miR-197-5P was also a risk factor for composite endpoint events in patients under the age of 50 years with stage C or D HF.

CONCLUSION

miR-197-5P is a circulation miRNA that correlates with MF and adverse cardiac events in HF patients under the age of 50 years.

摘要

目的

本研究旨在鉴定心力衰竭（HF）特异性循环微小RNA（miRNA），并检测所选miRNA是否与心肌纤维化相关，以及是否反映C期或D期HF患者不良心血管事件的发生率。

方法

通过微阵列分析检测终末期HF患者和匹配的健康对照中表达的循环miRNA，并通过定量实时聚合酶链反应进行验证。进行多变量Cox回归分析，以确定所选循环miRNA是否可能是HF患者的预后因素。

结果

在7名健康对照和9名C期或D期HF患者的队列中，有7种miRNA差异表达。在另一组80名C期或D期HF患者和30名健康对照中对这些miRNA进行了进一步研究。只有miR-197-5P与50岁以下C期或D期HF患者心脏磁共振成像中所见的纤维化相关（r = 0.42，p = 0.008）。多变量分析显示，miR-197-5P也是50岁以下C期或D期HF患者复合终点事件的危险因素。