Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA.
Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO 80523-1692, USA.
Viruses. 2019 Mar 6;11(3):225. doi: 10.3390/v11030225.
The global burden of arboviral diseases and the limited success in controlling them calls for innovative methods to understand arbovirus infections. Metabolomics has been applied to detect alterations in host physiology during infection. This approach relies on mass spectrometry or nuclear magnetic resonance spectroscopy to evaluate how perturbations in biological systems alter metabolic pathways, allowing for differentiation of closely related conditions. Because viruses heavily depend on host resources and pathways, they present unique challenges for characterizing metabolic changes. Here, we review the literature on metabolomics of arboviruses and focus on the interpretation of identified molecular features. Metabolomics has revealed biomarkers that differentiate disease states and outcomes, and has shown similarities in metabolic alterations caused by different viruses (e.g., lipid metabolism). Researchers investigating such metabolomic alterations aim to better understand host⁻virus dynamics, identify diagnostically useful molecular features, discern perturbed pathways for therapeutics, and guide further biochemical research. This review focuses on lessons derived from metabolomics studies on samples from arbovirus-infected humans.
虫媒病毒病的全球负担以及控制这些疾病的有限成效，呼吁我们采用创新方法来了解虫媒病毒感染。代谢组学已被应用于检测感染过程中宿主生理的变化。这种方法依赖于质谱或核磁共振波谱学来评估生物系统中扰动如何改变代谢途径，从而区分密切相关的情况。由于病毒严重依赖宿主资源和途径，因此它们在表征代谢变化方面提出了独特的挑战。在这里，我们回顾了关于虫媒病毒代谢组学的文献，并重点介绍了对鉴定出的分子特征的解释。代谢组学揭示了可区分疾病状态和结果的生物标志物，并显示了不同病毒引起的代谢改变的相似性（例如，脂代谢）。研究人员研究这些代谢变化的目的是更好地了解宿主-病毒动态，识别具有诊断用途的分子特征，辨别受干扰的治疗途径，并指导进一步的生化研究。本综述重点介绍了从感染虫媒病毒的人类样本的代谢组学研究中获得的经验教训。
