Monteiro Diana C F, Vakili Mohammad, Harich Jessica, Sztucki Michael, Meier Susanne M, Horrell Sam, Josts Inokentijs, Trebbin Martin
The Hamburg Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, Hamburg 22761, Germany.
ESRF, European Synchrotron Radiation Facility, 71 Avenue des Martyrs, Grenoble CS 40220, France.
J Synchrotron Radiat. 2019 Mar 1;26(Pt 2):406-412. doi: 10.1107/S1600577519000304. Epub 2019 Feb 13.
Serial synchrotron crystallography allows low X-ray dose, room-temperature crystal structures of proteins to be determined from a population of microcrystals. Protein production and crystallization is a non-trivial procedure and it is essential to have X-ray-compatible sample environments that keep sample consumption low and the crystals in their native environment. This article presents a fast and optimized manufacturing route to metal-polyimide microfluidic flow-focusing devices which allow for the collection of X-ray diffraction data in flow. The flow-focusing conditions allow for sample consumption to be significantly decreased, while also opening up the possibility of more complex experiments such as rapid mixing for time-resolved serial crystallography. This high-repetition-rate experiment allows for full datasets to be obtained quickly (∼1 h) from crystal slurries in liquid flow. The X-ray compatible microfluidic chips are easily manufacturable, reliable and durable and require sample-flow rates on the order of only 30 µl h.
串行同步加速器晶体学能够在低X射线剂量下，从微晶群体中确定蛋白质在室温下的晶体结构。蛋白质的生产和结晶是一个复杂的过程，拥有与X射线兼容的样品环境至关重要，这种环境能保持低样品消耗，并使晶体处于其天然环境中。本文介绍了一种快速且优化的制造金属-聚酰亚胺微流控流动聚焦装置的路线，该装置能够在流动状态下收集X射线衍射数据。流动聚焦条件可显著降低样品消耗，同时也为更复杂的实验提供了可能性，例如用于时间分辨串行晶体学的快速混合实验。这种高重复率实验能够从液流中的晶体浆液快速（约1小时）获得完整数据集。与X射线兼容的微流控芯片易于制造、可靠且耐用，所需样品流速仅为30 μl/h左右。
