Pietersen G, Staples M S, Kasdorf G G F, Thomas J E
ARC-Plant Protection Research Institute (PPRI), Private Bag X134, Pretoria, 0001.
Queensland Department of Primary Industries, 80 Meiers Road, Indooroopilly, Queensland 4068, Australia.
Plant Dis. 1998 Oct;82(10):1171. doi: 10.1094/PDIS.1998.82.10.1171B.
Cucumber mosaic cucumovirus (CMV) is the etiological agent of infectious chlorosis disease in bananas (Musa spp.). In South Africa, diagnosis of CMV on banana has been based only on symptoms (1). A Grande Naine (Cavendish, AAA) plant with typical infectious chlorosis disease was obtained from Letsitele. Sap from this plant was inoculated to indicator plants. Virus was isolated by two serial local lesion transfers on Chenopodium quinoa and maintained on Nicotiana benthamiana. It was identified as a subgroup I CMV based on its reaction to CMV DTL-, but not CMV ToRS- monoclonal antibodies in double antibody sandwich (DAS)-enzyme-linked immunosorbent assay (ELISA). The identity was further confirmed by the polyhedral particle morphology under the electron microscope by negatively staining, and decoration in immunoelectron microscopy with antiserum to a local tomato isolate of CMV. Infectious chlorosis disease symptoms were induced by sap inoculation to healthy Grande Naine plants, and the virus detected by ELISA. This is the first confirmed diagnosis of CMV on bananas in South Africa. The isolate was deposited in the PPRI Virus Collection. Reference: (1) B. Q. Manicom. 1993. Pages 102-103 in: Handbook of banana growing in South Africa. J. C. Robinson, ed. Agric. Res. Counc., Inst. Trop. Subtrop.Crops, Nelspruit, South Africa.
黄瓜花叶黄瓜病毒（CMV）是香蕉（芭蕉属）传染性褪绿病的病原体。在南非，香蕉上CMV的诊断仅基于症状（1）。从莱齐泰莱获得了一株患有典型传染性褪绿病的大密哈（卡文迪什，AAA）植株。将该植株的汁液接种到指示植物上。通过在藜上进行两次连续的局部病斑转移分离病毒，并在本氏烟草上保存。在双抗体夹心（DAS）-酶联免疫吸附测定（ELISA）中，根据其对CMV DTL -单克隆抗体而非CMV ToRS -单克隆抗体的反应，将其鉴定为I亚组CMV。通过负染色在电子显微镜下观察多面体颗粒形态，并在免疫电子显微镜中用针对CMV番茄本地分离株的抗血清进行装饰，进一步确认了其身份。通过将汁液接种到健康的大密哈植株上诱导出传染性褪绿病症状，并通过ELISA检测到病毒。这是南非首次确诊香蕉上的CMV。该分离株保存在PPRI病毒保藏中心。参考文献：（1）B. Q. Manicom。1993年。载于：《南非香蕉种植手册》。J. C. Robinson编。农业研究理事会，热带亚热带作物研究所，南非内尔斯普雷特。
