Otsuka T, Villaret D, Yokota T, Takebe Y, Lee F, Arai N, Arai K
Nucleic Acids Res. 1987 Jan 12;15(1):333-44. doi: 10.1093/nar/15.1.333.
Based on homology with the mouse interleukin 4 (IL-4) cDNA that expresses B cell, T cell, and mast cell stimulating activities (Lee, F. et al., (1986) Proc. Natl. Acad. Sci. USA 83, 2061), we have isolated from a Balb/c mouse liver DNA library the mouse chromosomal gene and analyzed its overall structure. The gene occurs as a single copy in the haploid genome and contains four exons and three introns. The exon sequences almost completely match the cloned cDNA sequence. Interestingly, there is a fairly high degree of homology between mouse IL-4 and mouse IL-2 genes extending more than 200 bp upstream of a "TATA" like sequence located 20 bp upstream of the transcription initiation site. These sequences may play an important role in the regulated expression of this gene in concanavalin A or antigen-stimulated T lymphocytes. The supernatant of COS7 cells transfected with plasmid DNA containing the entire gene exhibited both T cell growth factor and mast cell growth factor activities.
基于与表达B细胞、T细胞和肥大细胞刺激活性的小鼠白细胞介素4（IL-4）cDNA的同源性（Lee, F.等人，(1986) 《美国国家科学院院刊》83, 2061），我们从Balb/c小鼠肝脏DNA文库中分离出小鼠染色体基因并分析了其整体结构。该基因在单倍体基因组中以单拷贝形式存在，包含四个外显子和三个内含子。外显子序列几乎与克隆的cDNA序列完全匹配。有趣的是，小鼠IL-4和小鼠IL-2基因之间存在相当高的同源性，这种同源性在转录起始位点上游20 bp处的类似“TATA”序列上游延伸超过200 bp。这些序列可能在伴刀豆球蛋白A或抗原刺激的T淋巴细胞中该基因的调控表达中发挥重要作用。用含有完整基因的质粒DNA转染的COS7细胞的上清液表现出T细胞生长因子和肥大细胞生长因子活性。
